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Hub AI
Luxol fast blue stain AI simulator
(@Luxol fast blue stain_simulator)
Hub AI
Luxol fast blue stain AI simulator
(@Luxol fast blue stain_simulator)
Luxol fast blue stain
Luxol fast blue stain, abbreviated LFB stain or simply LFB, is a commonly used stain to observe myelin under light microscopy, first developed by Heinrich Klüver and Elizabeth Barrera in 1953. Luxol fast blue refers to one of a group of three chemically and histologically similar dyes. LFB is commonly used to detect demyelination in the central nervous system (CNS), but cannot well discern myelination in the peripheral nervous system.
Luxol fast blue dyes were produced by DuPont since at least 1944. Luxol refers to the original trade name used first by DuPont, and later, the Rohm & Haas division of Dow Chemical. Du Pont produced three blue dyes sold under the Luxol trade name, in addition to various other "fast" dyes. The first method of using a luxol fast blue was described by Klüver and Barrera in 1953.
There are three types of luxol fast blue: luxol fast blue MBS, luxol fast blue ARN, and luxol fast blue G. LFB MBS is the original and most widely used luxol stain, and was the stain used by Klüver and Barrera. Researchers have since developed similar stain protocols using luxol fast blue ARN.
LFB MBS is the bis[1,3-di(2-tolyl)guanidinium] salt of a copper phthalocyanine-disulfonic acid. The chemical formula for the MBS dye is ; the acid, known as luxol fast blue MBS free acid, has the chemical formula . LFB MBS is a phthalocyanine dye. LFB ARN and LFB G, by contrast, are diarylguanidine salts of sulphonated azo dyes. LFB ARN is better known as anazolene sodium, with the chemical formula . LFB G has the formula .
![{\displaystyle {\mathrm {C} {\vphantom {A}}_{\smash[{t}]{32}}\mathrm {H} {\vphantom {A}}_{\smash[{t}]{14}}\mathrm {CuN} {\vphantom {A}}_{\smash[{t}]{8}}\mathrm {Na} {\vphantom {A}}_{\smash[{t}]{2}}\mathrm {O} {\vphantom {A}}_{\smash[{t}]{6}}\mathrm {S} {\vphantom {A}}_{\smash[{t}]{2}}}}](https://wikimedia.org/api/rest_v1/media/math/render/svg/4804848558ccebf9e637352e33b741c55c6fd107)
![{\displaystyle {[\mathrm {C} {\vphantom {A}}_{\smash[{t}]{32}}\mathrm {H} {\vphantom {A}}_{\smash[{t}]{16}}\mathrm {CuN} {\vphantom {A}}_{\smash[{t}]{8}}\mathrm {O} {\vphantom {A}}_{\smash[{t}]{6}}\mathrm {S} {\vphantom {A}}_{\smash[{t}]{2}}]{\vphantom {A}}^{2-}}}](https://wikimedia.org/api/rest_v1/media/math/render/svg/ea37e48b254e856e1df76108a0d3f4a1b4431689)
![{\displaystyle {\mathrm {C} {\vphantom {A}}_{\smash[{t}]{26}}\mathrm {H} {\vphantom {A}}_{\smash[{t}]{16}}\mathrm {N} {\vphantom {A}}_{\smash[{t}]{3}}\mathrm {Na} {\vphantom {A}}_{\smash[{t}]{3}}\mathrm {O} {\vphantom {A}}_{\smash[{t}]{10}}\mathrm {S} {\vphantom {A}}_{\smash[{t}]{3}}}}](https://wikimedia.org/api/rest_v1/media/math/render/svg/f9f901bd8a9b21a955fe3ec142f7508988a41931)
![{\displaystyle {\mathrm {C} {\vphantom {A}}_{\smash[{t}]{57}}\mathrm {H} {\vphantom {A}}_{\smash[{t}]{46}}\mathrm {N} {\vphantom {A}}_{\smash[{t}]{10}}\mathrm {O} {\vphantom {A}}_{\smash[{t}]{13}}\mathrm {S} {\vphantom {A}}_{\smash[{t}]{4}}}}](https://wikimedia.org/api/rest_v1/media/math/render/svg/2b6d94b931c9b7f4908b2cd293dd431d77bfaf33)
Luxol fast blue is used primarily to stain the myelin sheaths of neurons. Luxol fast blues undergoes an acid-base reaction to bind to the bases of phospholipids; while the exact bases involved are unknown, previous research has shown strong affinities towards the phospholipids phosphotidyl choline, phosphotidyl ethanolamine, phosphotidyl serine, and sphingomyelin. Together, these phosphoglycerides make up 27.6% of the dry weight of isolated myelin. The various luxol fast blues are histologically similar, with only minor variations in affinity towards certain phospholipids.
In the staining procedure, tissue sections are stained with a solution consisting of one of the luxol fast blues and ethanol (sometimes, glacial acetic acid is added). There are two main LFB staining protocols: conventional LFB staining and the MCOLL protocol, and are primarily performed on paraffin sections.
A typical conventional LFB staining is performed as follows:
In the MCOLL protocol, the following steps are added after differentiation is stopped, and before the transfer to xylene and mounting:
Luxol fast blue stain
Luxol fast blue stain, abbreviated LFB stain or simply LFB, is a commonly used stain to observe myelin under light microscopy, first developed by Heinrich Klüver and Elizabeth Barrera in 1953. Luxol fast blue refers to one of a group of three chemically and histologically similar dyes. LFB is commonly used to detect demyelination in the central nervous system (CNS), but cannot well discern myelination in the peripheral nervous system.
Luxol fast blue dyes were produced by DuPont since at least 1944. Luxol refers to the original trade name used first by DuPont, and later, the Rohm & Haas division of Dow Chemical. Du Pont produced three blue dyes sold under the Luxol trade name, in addition to various other "fast" dyes. The first method of using a luxol fast blue was described by Klüver and Barrera in 1953.
There are three types of luxol fast blue: luxol fast blue MBS, luxol fast blue ARN, and luxol fast blue G. LFB MBS is the original and most widely used luxol stain, and was the stain used by Klüver and Barrera. Researchers have since developed similar stain protocols using luxol fast blue ARN.
LFB MBS is the bis[1,3-di(2-tolyl)guanidinium] salt of a copper phthalocyanine-disulfonic acid. The chemical formula for the MBS dye is ; the acid, known as luxol fast blue MBS free acid, has the chemical formula . LFB MBS is a phthalocyanine dye. LFB ARN and LFB G, by contrast, are diarylguanidine salts of sulphonated azo dyes. LFB ARN is better known as anazolene sodium, with the chemical formula . LFB G has the formula .
Luxol fast blue is used primarily to stain the myelin sheaths of neurons. Luxol fast blues undergoes an acid-base reaction to bind to the bases of phospholipids; while the exact bases involved are unknown, previous research has shown strong affinities towards the phospholipids phosphotidyl choline, phosphotidyl ethanolamine, phosphotidyl serine, and sphingomyelin. Together, these phosphoglycerides make up 27.6% of the dry weight of isolated myelin. The various luxol fast blues are histologically similar, with only minor variations in affinity towards certain phospholipids.
In the staining procedure, tissue sections are stained with a solution consisting of one of the luxol fast blues and ethanol (sometimes, glacial acetic acid is added). There are two main LFB staining protocols: conventional LFB staining and the MCOLL protocol, and are primarily performed on paraffin sections.
A typical conventional LFB staining is performed as follows:
In the MCOLL protocol, the following steps are added after differentiation is stopped, and before the transfer to xylene and mounting:
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