Megamind vs. the Doom Syndicate is a 2024 American animated direct-to-streaming superhero comedy film produced by DreamWorks Animation Television and directed by Eric Fogel. It is the sequel to the 2010 film, Megamind, in addition to serving as the pilot for the television series, Megamind Rules!, which premiered the same day. Starring Keith Ferguson, Laura Post, and Josh Brener, the film follows Megamind, now a superhero, as he protects Metro City from his former allies in the Doom Syndicate, a team of supervillains.

Megamind vs. the Doom Syndicate was released on Peacock on March 1, 2024, and received negative reviews from critics.

Two days after the events of the first film, Megamind now assumes the role of Metro City's defender. He, along with Ol' Chum (formerly Minion), stops a criminal trio known as the Go-Fish gang from stealing a priceless fish. For his heroic efforts, Megamind is presented the key to the city. Chum, feeling left out and undervalued in his efforts, tells Megamind that he wants to be his sidekick. Upon being rejected, Chum claims that he will search for other opportunities, which Megamind allows.

The Doom Syndicate, a supervillain team consisting of Lady Doppler, Pierre Pressure, Behemoth, and Lord Nighty-Knight, which Megamind used to be part of, breaks out of prison to visit him, thinking that his hero role is part of an evil plan. They embark on a crime spree, eventually challenging Megamind to prove he is still evil by robbing a bank. Megamind struggles to do so, but is helped by Keiko Morita, his self-proclaimed biggest fan, who helps him stage a fake robbery. Meanwhile, Chum has taken up work at a run-down diner.

Keeping his old crew fooled, Megamind reveals an abandoned villainous plot he conducted of launching Metro City to the Moon using rockets planted underground. The Doom Syndicate prepares to launch them, but Megamind stalls them. They suspect he is up to something, and vow to either launch Metro City themselves or destroy it. Realizing that he cannot defeat the Syndicate alone, Megamind tracks Chum inside his remodeled diner and attempts to enlist his help. However, after realizing he might belong more at the shop than with him, Megamind regrets letting Chum go.

Megamind finds himself overpowered by the Doom Syndicate after they learn of his facade. He hides in an alleyway, feeling defeated, but is confronted by Roxanne and Keiko, who remind him it is okay to ask for help from others. Chum also returns as Megamind tells him he is truly his sidekick. A rejuvenated Megamind, along with Chum, Roxanne devise a plan save the day and appoint Keiko as part of their crew. They make a quick stop to Everything City to gather supplies for their plan while the Doom Syndicate struggles to open the door to the control room.

Upon breaking in, Lady Doppler presses the button to initiate the launch. Megamind and Roxanne face off against the Doom Syndicate to buy time for Chum to stop the launch, but are unsuccessful. During the City's ascent, Chum terminates the launch, but Metro City is now falling at a dangerous rate. Megamind leads the Doom Syndicate to a construction site, where, with the help of Roxanne and Keiko, defeats the villains one by one. The crew manages to safely redirect Metro City back to Earth. Roxanne is elected the new Mayor of Metro City, while Keiko launches a hero signal as Megamind feels accomplished in his new team.

In a mid-credits scene, the Doom Syndicate are locked back up in prison, where they are visited by Megamind's former mentor, Machiavillain.