The Ministry of Internal Affairs of Ukraine (Ukrainian: Міністерство внутрішніх справ України, romanized: Ministerstvo vnutrishnikh sprav Ukrainy; pronounced [min⁽ʲ⁾iˈstɛrstwo ˈu̯nut⁽ʲ⁾r⁽ʲ⁾iʃn⁽ʲ⁾ix ˈsprɑu̯ ʊkrɐˈjinɪ]; MVS) is the ministry of the Ukrainian government that oversees the interior affairs of Ukraine.

Formerly, the ministry directly controlled the Ukrainian national law enforcement agency, termed the militsiya (Ukrainian: міліція, Russian: милиция).

This changed in July 2015, in the aftermath of Euromaidan, with the introduction of reforms by Ukrainian president Petro Poroshenko to reduce corruption, whereby the militsiya was replaced with the National Police.

Ukraine's militsiya was widely regarded as corrupt, and it had received accusations of torture and ill-treatment.

The State Emergency Service was transferred under the jurisdiction of the ministry since 2014.

The ministry carries out state policy for the protection of rights and liberties of citizens, investigates unlawful acts against the interest of society and state, fights crime, provides civil order, ensures civil security and traffic safety, and guarantees the security and protection of important individuals.

It is a centralised agency headed by the Minister of Internal Affairs. The ministry works closely with the office of the General Prosecutor of Ukraine.

It oversees the National Police of Ukraine (police service), National Guard of Ukraine (gendarmerie), the State Emergency Service of Ukraine (civil defense), State Border Guard Service of Ukraine (and its subordinate the Ukrainian Sea Guard) and the State Migration Service (border control service).