Miss Ukraine Universe (Ukrainian: Міс Всесвіт Україна, romanized: Mis Vsesvit Ukraina) is a national beauty pageant in Ukraine. The winner represents Ukraine at Miss Universe.

The pageant has existed since 2006, after the Miss Ukraine Organization lost the Miss Universe franchise to Oleksandra Nikolayenko. The winner of the pageant represents Ukraine at Miss Universe. Previously, the representative of Ukraine was chosen by the Miss Ukraine Committee.

The current Miss Ukraine Universe is Sofiya Tkachuk from Rivne, who was crowned on 9 June 2025.

The Miss Ukraine Universe pageant was founded in 2006 by Oleksandra Nikolayenko.

Natalie Glebova, Miss Universe 2005 was invited by Nikolayenko to visit Ukraine to promote the Miss Ukraine Universe contest in 2006. The inaugural contest was won by Inna Tsymbalyuk.

In April 2016 Nikolayenko sold the pageant. A foreign-owned international company became the new owner of the Miss Ukraine Universe beauty contest.

The current Miss Universe license holder in Ukraine and the National Director of the pageant is Anna Filimonova.

Participants of the pageant should be citizens of Ukraine and have from 18 to 28 years of age.