Nitronate monooxygenase

Nitronate monooxygenase (EC 1.13.12.16, NMO) is an enzyme with systematic name nitronate:oxygen 2-oxidoreductase (nitrite-forming).^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

\rightleftharpoons

acetaldehyde + nitrite + other products

The enzymes from the fungus Neurospora crassa and the yeast Williopsis saturnus var. mrakii contain non-covalently bound FMN as the cofactor.

References

^ Francis K, Russell B, Gadda G (February 2005). "Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzed by 2-nitropropane dioxygenase". The Journal of Biological Chemistry. 280 (7): 5195–204. doi:10.1074/jbc.M411249200. PMID 15582992.
^ Ha JY, Min JY, Lee SK, Kim HS, Kim DJ, Kim KH, Lee HH, Kim HK, Yoon HJ, Suh SW (July 2006). "Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base". The Journal of Biological Chemistry. 281 (27): 18660–7. doi:10.1074/jbc.M601658200. PMID 16682407.
^ Gadda G, Francis K (January 2010). "Nitronate monooxygenase, a model for anionic flavin semiquinone intermediates in oxidative catalysis". Archives of Biochemistry and Biophysics. 493 (1): 53–61. doi:10.1016/j.abb.2009.06.018. PMID 19577534.
^ Francis K, Gadda G (October 2009). "Kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase". Bioorganic Chemistry. 37 (5): 167–72. doi:10.1016/j.bioorg.2009.07.005. PMID 19683782.

Nitronate+monooxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Oxidoreductases: monooxygenases (EC 1.13)
1.13.11: two atoms of oxygen	lipoxygenase: Arachidonate 5-lipoxygenase Arachidonate 12-lipoxygenase/ALOX12 Arachidonate 8-lipoxygenase Arachidonate 15-lipoxygenase/ALOX15 Linoleate 11-lipoxygenase other dioxygenase: Catechol dioxygenase Homogentisate 1,2-dioxygenase Cysteine dioxygenase 4-Hydroxyphenylpyruvate dioxygenase Indoleamine 2,3-dioxygenase Chlorite dismutase
1.13.12: one atom of oxygen	Firefly luciferase
1.13.99: other	Inositol oxygenase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)