The ossicles (also called auditory ossicles) are three irregular bones in the middle ear of humans and other mammals, and are among the smallest bones in the human body. Although the term "ossicle" literally means "tiny bone" (from Latin ossiculum) and may refer to any small bone throughout the body, it typically refers specifically to the malleus, incus and stapes ("hammer, anvil, and stirrup") of the middle ear.

The auditory ossicles serve as a kinematic chain to transmit and amplify (intensify) sound vibrations collected from the air by the ear drum to the fluid-filled labyrinth (cochlea). The absence or pathology of the auditory ossicles would constitute a moderate-to-severe conductive hearing loss.

The ossicles are, in order from the eardrum to the inner ear (from superficial to deep): the malleus, incus, and stapes, terms that in Latin are translated as "the hammer, anvil, and stirrup".

Studies have shown that ear bones in mammal embryos are attached to the dentary, which is part of the lower jaw. These are ossified portions of cartilage—called Meckel's cartilage—that are attached to the jaw. As the embryo develops, the cartilage hardens to form bone. Later in development, the bone structure breaks loose from the jaw and migrates to the inner ear area. The structure is known as the middle ear, and is made up of the stapes, incus, malleus, and tympanic membrane. These correspond to the columella, quadrate, articular, and angular structures in the amphibian, bird or reptile jaw.

As sound waves vibrate the tympanic membrane (eardrum), it in turn moves the nearest ossicle, the malleus, to which it is attached. The malleus then transmits the vibrations, via the incus, to the stapes, and so ultimately to the membrane of the fenestra ovalis (oval window), the opening to the vestibule of the inner ear.

Sound traveling through the air is mostly reflected when it comes into contact with a liquid medium; only about 1/30 of the sound energy moving through the air would be transferred into the liquid. This is observed from the abrupt cessation of sound that occurs when the head is submerged underwater. This is because the relative incompressibility of a liquid presents resistance to the force of the sound waves traveling through the air. The ossicles give the eardrum a mechanical advantage via lever action and a reduction in the area of force distribution; the resulting vibrations are stronger but don't move as far. This allows more efficient coupling than if the sound waves were transmitted directly from the outer ear to the oval window. This reduction in the area of force application allows a large enough increase in pressure to transfer most of the sound energy into the liquid. The increased pressure will compress the fluid found in the cochlea and transmit the stimulus. Thus, the lever action of the ossicles changes the vibrations so as to improve the transfer and reception of sound, and is a form of impedance matching.

However, the extent of the movements of the ossicles is controlled (and constricted) by two muscles attached to them (the tensor tympani and the stapedius). It is believed that these muscles can contract to dampen the vibration of the ossicles, in order to protect the inner ear from excessively loud noise (theory 1) and that they give better frequency resolution at higher frequencies by reducing the transmission of low frequencies (theory 2) (see acoustic reflex). These muscles are more highly developed in bats and serve to block outgoing cries of the bats during echolocation (SONAR).

Occasionally the joints between the ossicles become rigid. One condition, otosclerosis, results in the fusing of the stapes to the oval window. This reduces hearing and may be treated surgically using a passive middle ear implant.^{[further explanation needed]}