The President's Dining Room is a dining room located in the northwest corner of the second floor of the White House. It is located directly above the Family Dining Room on the State Floor and looks out upon the North Lawn. The Dining Room is adjacent to the Family Kitchen, a small kitchen designed for use by the First Family, and served by a dumbwaiter connected to the main kitchen on the ground floor.

Beginning in the 19th century the space was occupied by a bedroom suite known as the Prince of Wales Room, named for an 1860 stay by the then-Prince of Wales, Albert Edward. From 1929 to 1948, this suite was known as the Lincoln Bedroom, with furnishings acquired by First Lady Mary Todd Lincoln (the current Lincoln Bedroom is now down the hall, in what was Lincoln's office suite). The bedroom suite was structurally changed in 1961 to create a dining room and kitchen in the First Family's residence.

The President's Dining Room is located in the northwest corner of the Second Floor. When this part of the Executive Residence was completed in 1809, a bedroom suite occupied the space. What is now the private stairs and Cosmetology Room were the eastern chamber and closet of the suite, A bedroom and toilet occupied what is now the President's Dining Room, and a lady's dressing room was in the space currently occupied by the Family Kitchen, the kitchen storage space, and the pantry.

This space was little changed by 1825. Since the private stairs from the Ground Floor were now complete, the closet in the eastern chamber was removed and a landing for the stairs inserted in the middle of the room. This effectively created an open storage area in the southern third of the chamber. First Lady Louisa Adams and her niece, Mary Hellen, used the bedroom and dressing room as a bedroom suite (but did not occupy the eastern chamber). From March 1829 to the summer of 1830, all three rooms were occupied by Jack and Emily Donelson and their four children. President William Henry Harrison used the larger bedroom as his personal bedroom during his 32-day presidency in 1841. Robert Tyler (son of President John Tyler), his wife, and daughter used all three rooms from April 1841 to March 1845. From 1845 to 1849, the bedroom, dressing room, and eastern chamber were used by Augusta Tabb Walker and her two small children.

The bedroom suite became known as the Prince of Wales Room after Albert Edward, Prince of Wales stayed in the room in 1860. Although a full bath was added to the eastern chamber, there was no connecting door between it and the bedroom. To accommodate the bathroom, the stairs were moved from the middle to the southern part of the room, and the storage space eliminated.

Mary Todd Lincoln's refurbishment of the White House in 1861 led to historic changes in the room. Mrs. Lincoln purchased two armchairs, a rosewood center table, a chest of drawers, four side balloon-back side chairs, a sofa, and—most importantly—a 6-foot (1.8 m) wide, 8-foot (2.4 m) long rosewood bed frame for the room. The headboard was pierced and richly carved with images of birds, grapes, and vines. The footboard featured similar, though more simply-carved, images. Attached to the headboard was a gilt canopy carved in the shape of a crown, with a shield in the front. Purple satin trimmed in gold lace hung from the canopy. This bed became known as the "Lincoln bed", even though President Lincoln is not known to have slept in it. Mrs. Lincoln also purchased a Wilton carpet to cover the floor, and purple-tinted French velvet wallpaper with crimson stripes and repetitive golden images of a moss rose tree in bloom.

In this configuration, the bedroom was used by young Willie Lincoln, who died of fever in this room in 1862. On April 16, 1865, Dr. Janvier Woodward and Dr. Edward Curtis autopsied, and Dr. Charles D. Brown embalmed, Abraham Lincoln in this room.

First Lady Eliza Johnson used the small eastern chamber as her bedroom, while the First Family used the larger bedroom (later to be the President's Dining Room) as a living room. The large room was later used as a bedroom by Nellie Grant (daughter of President Ulysses S. Grant); Fanny Hayes (daughter of President Rutherford B. Hayes); Mary "Mollie" Garfield (daughter of President James Garfield); Ellen "Nell" Arthur (daughter of President Chester Arthur); President Grover Cleveland and First Lady Frances Cleveland; James Robert and Mary Harrison McKee and their two small children; President William McKinley and his wife, Ida; Alice Roosevelt (daughter of President Theodore Roosevelt); Ethel Roosevelt (daughter of President Theodore Roosevelt); First Lady Helen Taft; Eleanor Wilson (daughter of President Woodrow Wilson); and Calvin Coolidge, Jr.;