Priscilla Lane (born Priscilla Mullican; June 12, 1915 – April 4, 1995) was an American actress, and the youngest sibling in the Lane Sisters' family of singers and actresses. She is best remembered for her roles in the films The Roaring Twenties (1939) co-starring with James Cagney and Humphrey Bogart; Saboteur (1942), an Alfred Hitchcock film in which she plays the heroine; and Arsenic and Old Lace (1944), in which she portrays Cary Grant's fiancée and bride.

Priscilla Mullican was born on June 12, 1915, in Indianola, Iowa, a small college town south of Des Moines. She was the youngest of five daughters of Lorenzo Mullican and his wife, Cora Bell Hicks. Mullican had a dental practice in Indianola. The family owned a large house with 22 rooms, some of which they rented to students attending nearby Simpson College.

Priscilla and one of her sisters, Rosemary, traveled to Des Moines every weekend to study dancing with Rose Lorenz, a renowned dance teacher at the time. The girls made their first professional appearance September 30, 1930, at Des Moines' Paramount Theater. Priscilla, then 15, performed on stage as part of the entertainment accompanying the release of her sister Lola's Hollywood movie Good News (1930).

After graduating from high school, Priscilla was permitted to travel to New York to visit a third sister, Leota (the eldest of the five Mullican sisters), then appearing in a musical revue in Manhattan. Priscilla enrolled at the nearby Fagen School of Dramatics, and Leota paid the fee. At this time, talent agent Al Altman saw Priscilla performing in one of Fagen's school plays and invited her to screentest for MGM. She was 16 years old. Priscilla wrote to a friend in Indianola, "Leota accompanied me to a sort of theater in a New York skyscraper. Others were there being made up. One was a strange looking girl with her hair slicked back in a sort of a bun. Her name is said to be Catherine Hepburn [sic]. Not very pretty, I thought, but Mr. Altman said she has something. Margaret Sullavan, the Broadway actress, was there too!" A follow-up letter said that her test had proven unsuitable. Neither Hepburn nor Sullavan was approved, and neither received a contract from MGM at the time.

In 1932, Cora and Rosemary arrived in New York. Cora immediately went to work pushing her two young daughters into attending auditions for various prospective Broadway productions, without success. During a tryout at a music publishing office, orchestra leader and radio personality Fred Waring heard them harmonizing. He found them attractive and talented and soon signed them to a radio contract. Priscilla, who at this time adopted the surname Lane, quickly became known as the comedienne of the group. Rosemary sang the ballads, while Priscilla performed the swing numbers and wisecracked with Waring and various guests. Back in Iowa, Mullican instituted divorce proceedings against his wife on the grounds of desertion, and the divorce was granted in 1933.

Rosemary and Priscilla remained with Fred Waring for almost five years. In 1937, Waring was engaged by Warner Bros. in Hollywood to appear with his entire band in Varsity Show, a musical starring Dick Powell. Both Rosemary and Priscilla were tested and awarded feature roles in the film. Rosemary shared the romantic passages with Powell, while Priscilla played a high-spirited college girl.

Warner Bros. purchased Priscilla and Rosemary's contract from Fred Waring and signed them to seven-year pacts. Priscilla's first film after Varsity Show was Men Are Such Fools (1938), in which she starred with Wayne Morris. This was followed by Love Honor and Behave (also 1938), another light romantic comedy with Morris, who, playing her husband, spanked her 47 times in a scene for which she declined a double, and Cowboy from Brooklyn, again teaming with Dick Powell. The publicity department at the studio suggested that Priscilla and Morris be seen together around town. They liked each other and did date for a period; however, Priscilla later said it was never serious on either side.

Also in 1938, Priscilla, Rosemary and third sister Lola Lane appeared as three of four sisters (the fourth being Gale Page) in Four Daughters, the similarly themed Daughters Courageous the following year, and two sequels to Four Daughters a few years later, Four Wives and Four Mothers.