The Queen Sofía Spanish Institute is an organization in New York City, founded with the aim of promoting the culture of Spanish speaking countries and to foster their relations with the United States. It is located at the Oliver D. Filley House on the Upper East Side of Manhattan.

On May 18, 1954, the institute was incorporated as a non-political, non-profit organization under the laws of the State of New York.

In 1965, the philanthropist Margaret Rockefeller Strong, saved the landmark building 684 Park Avenue from destruction. With her support and funding from McMicking Foundation, the institute made this building its headquarters.

The institute was renamed in 2003, to recognize Queen Sofía of Spain as its patroness.

In 2014, the building was sold. The institute cosponsors lectures, educational programs and other culturally relevant activities.

In 2018, the Institute formed a partnership with the Hispanic Society to provide a Maps and Globes educational program in New York City Public Schools of District 6. Other partners of the Institute include the Instituto Cervantes, Hunter College, Juilliard, the Spanish Consulate and the Americas Society.

In June 2025, the institute was awarded the President General's Medallion by the Daughters of the American Revolution. The medallion was presented to the institute's president and CEO, Begonia Santos, by President General Pamela Rouse Wright during the opening night ceremony of the 134th DAR Continental Congress at DAR Constitution Hall in Washington, D.C.

Queen Sofía Spanish Institute, Inc. (the “Institute”) is a non-profit corporation founded in 1954 to stimulate American's interest in the Art, Culture, Customs, language, literature and history of the Spanish speaking world and to promote, among the Spanish-speaking peoples of the world; knowledge and understanding of ideals, culture and customs of the people of the United States, to the end that ties of friendship may be formed, mutual understanding promoted, and bonds of peace strengthened.