Queens Botanical Garden is a botanical garden located at 43-50 Main Street in the Flushing neighborhood of Queens in New York City, United States. The 39-acre (16 ha) site features rose, bee, herb, wedding, and perennial gardens; an arboretum; an art gallery; and a LEED-certified Visitor & Administration Building. Queens Botanical Garden is located on property owned by the City of New York, and is funded from several public and private sources. It is operated by Queens Botanical Garden Society, Inc.

Queens Botanical Garden was created as part of the 1939 New York World's Fair and was originally located in nearby Flushing Meadows–Corona Park. It moved to its current location, a landfilled area east of Flushing Meadows Park, in 1963 in preparation for the 1964 New York World's Fair. Since then, the Queens Botanical Garden has continued to expand, with programming targeted at residents of surrounding community. In 2001 the Queens Botanical Garden Society published a master plan for a renovation of the garden, centered around the garden's location above the underground Kissena Creek. Several improvements were made over the following years, including the construction of a new environmentally friendly parking lot and administration building.

During the 1939 New York World's Fair, held in adjacent Flushing Meadows–Corona Park, the future site of the Queens Botanical Garden was a horticultural exhibit of the fair called "Gardens on Parade" operated by Hortus, Incorporated. The original gardens were located just west of the modern site at the northeast corner of the fair grounds, at 131st Street between Lawrence Street and the Flushing River in the path of the future Van Wyck Expressway.

A New York City Department of Sanitation garage at Dahlia Avenue was located west of Main Street, in the modern Queens Botanical Garden. By the 1950s it had been abandoned, and there were calls to demolish it.

A playground located at Elder Avenue and 135th Street in what in now the Queens Botanical Garden, was originally set to be complete by March 1957. However, by March 11 only a comfort station and lights were constructed, while the site required significant filling before development could occur. According to the Parks Department, the project was delayed due to bad weather. The playground site was used as dumping ground, and it was filled with dirt after community petition. After a three-month delay, the playground was completed in June 1957.

Prior to the 1964–1965 New York World's Fair, the western portion of Kissena Corridor Park between Lawrence Street / College Point Boulevard and Main Street adjacent to Flushing Meadows Park was leased to the World's Fair Corporation, along with most of Flushing Meadows. In 1961, as part of a $3 million development for the World's Fair, the Queens Botanical Garden was planned to be relocated from the fair grounds in Flushing Meadows to a site across College Point Boulevard to the east, within the current Kissena Corridor Park and adjacent to the World's Fair grounds. This tract was described as "35 acres (14 ha) of bogs and dump land". The project included a new administration building, to cost $150,000, and a pedestrian overpass over Lawrence Street leading to Flushing Meadows. The existing garden in Flushing Meadows would be demolished to make way for new fair exhibits, and the extension of the Van Wyck Expressway north through the park to the Whitestone Expressway. This site was originally planned to be used as parking space for the fair.

Grading work for the project began on March 22, 1961. The Board of Estimate approved the Botanical Garden project and other World's Fair projects on September 23, 1961. At the time, the work for the gardens was estimated to cost $341,700. Construction on the administration building began in 1962. The building was designed by the Brodsky, Hopf & Adler firm, which also designed terminals at Dallas/Fort Worth International Airport in Texas. Landscaping work was done by Gilmore David Clarke and Michael Rapuano, who also designed the original 1939 World's Fair grounds and the 1964 layout for the fair. The section of Elder Avenue that ran southwest across the Corridor Park site between Main Street and Peck Avenue was de-mapped to integrate the land into the Botanical Gardens. Three Blue Atlas Cedar trees were transplanted from the original garden site to the new main entrance on Main Street. The new Queens Botanical Garden was dedicated on October 19, 1963.

Following Queens Botanical Garden's completion, New York City Parks commissioner Robert Moses unveiled an expanded plan for Kissena Corridor. The plan also included the Queens Zoo, to be built adjacent to the Queens Botanical Garden and operated by the Queens Botanical Garden Society. The zoo was expected to be complete by spring 1967, but ultimately opened in October 1968.