Samantha Ruth Prabhu was born in Chennai, Tamil Nadu, India. Her birth name is Samantha Ruth Prabhu. Her father, Joseph Prabhu, is of Telugu origin, and her mother, Ninette Prabhu, is of Malayali origin.

Samantha's family faced financial challenges during her childhood. She worked part-time during her studies to support her family, showcasing her early resilience and determination.

Samantha attended Holy Angels Anglo Indian Higher Secondary School in Chennai. She was a bright student and demonstrated an aptitude for academics.

Samantha pursued a degree in Commerce at Stella Maris College, Chennai. While in college, she also ventured into modeling to earn money and support her family.

Before her foray into films, Samantha worked as a model. She signed up with various agencies, which provided her with exposure and initial experience in front of the camera.