The National Shrine of Our Lady of the Abandoned, also known as Santa Ana Church, is a Spanish colonial period Roman Catholic church located in the district of Santa Ana in Manila, Philippines. It is under the jurisdiction of the Archdiocese of Manila. The parish was established by the Franciscan missionaries in 1578 under the patronage of Saint Anne. The present stone church was constructed by Vicente Inglés from 1720 to 1725 and dedicated to its present patron, Our Lady of the Abandoned. The revered image of its patron was made in Valencia, Spain in 1713, and arrived in the Philippines in 1717.

The church houses two National Cultural Treasures declared by the National Museum of the Philippines: the Santa Ana Site Museum located in the convent patio, and the Camarín de la Virgen (“Dressing Room of the Virgin”). It was elevated to the rank of national shrine in 2021.

The town of Santa Ana was first established by the Spanish Franciscan missionaries in 1578, in a settlement beside a creek connecting to the Pasig River, which the local inhabitants referred to as Sapà in the ancient Tagalog kingdom of Namayan. Its first church, dedicated to their titular patroness Saint Anne, after the similar-sounding Namayan, was made of nipa and bamboo until a decree from the colonial government in 1599 permitted the religious order to build the church in stone. However, the construction came only around 1720 upon the direction of then parish priest of Santa Ana, Vicente Inglés, wherein a new site was chosen, which is the current location of the structure. Dedicated to Our Lady of the Abandoned (Nuestra Señora de los Desamparados), the building of the stone church began on September 12, 1720, with the laying of the cornerstone by Governor-General and Archbishop of Manila Francisco de la Cuesta. Construction took five years to finish, with the total cost amounting to ₱33,000 including the donation of ₱4,000 by the Governor-General.

In the early 1700s, Inglés went to Valencia, Spain. The friar had been very enamored of a famous image of Our Lady that had become a popular spiritual attraction in Valencia known as the Our Lady of the Abandoned, comforting those whose loved ones have died. While Inglés was in Valencia in 1713, he decided to have a copy of this image for Santa Ana Parish, which was in the process of being constructed near Manila. After reverently touching the copy to the original image, the friar brought the new replica with him back to the Philippines in 1717. The image has been venerated in Santa Ana since then. In time, the parish became known by present name of Our Lady of the Abandoned Parish, though Saint Anne still is honored with a statue of her and the Child Mary in the reredos niche directly above that of Our Lady of the Abandoned. Governor-General De la Cuesta offered his bastón de mando to the image, and proclaimed her Gobernadora de la Ciudad de Manila ("Governoress of the City of Manila").

Santa Ana Church also served as barracks for wounded American soldiers during the Philippine–American War in 1899. The church complex was not destroyed by any bombs during the Battle of Manila in 1945.

The Catholic Bishops' Conference of the Philippines approved the petition to elevate the church to a national shrine in January 2020. The solemn declaration was held on May 12, 2021, the church’s titular feast day.

The church structure follows the Baroque architectural style, utilizing adobe blocks in its wall surface finish to give an impression of massiveness. Its floor plan formed the shape of a Latin cross oriented in north–south direction, with the main entrance facing north. The church facade is divided into three levels by heavily molded string courses. The topmost level or the pediment is adorned with Vitruvian-scroll design on its raking cornice, and a central niche flanked with octagonal windows. The second level is composed of semicircular arched windows covered with stained glass. The lowermost level is the main entrance flanked with two niches containing the statues of Anne and Joachim, the parents of the Mary. The ornamentation is mostly found in the frames of lateral niches and the moldings in the cornices and the apertures. Attached to the right side of the structure next to the church facade is the four-floored octagonal bell tower, also made of adobe blocks and were embellished by heavy string courses and other ornamentation.

The baptistery or bautisterio is located at the base of the bell tower on the right side of the vestibule or entrance hall. Inside the small room contains the marble baptismal font and a Neoclassical retablo featuring the painting of the Baptism of Christ, framed by two Corinthian columns on each side. Conservation efforts on the Santa Ana church revealed that two paintings have actually adorned the retablo of the baptistery. The first painting one was done on wood, which depicted John the Baptist pouring water on Jesus Christ, the Holy Spirit (symbolized by the dove) together with cherubs above them, and two angels holding a red cloak to cover Jesus. The wooden painting was later covered with the painting on metal, which most likely dated around 1938. The second painting has much simpler depiction, composing of only St. John the Baptist, Jesus Christ and the Holy Spirit above them.