Scanning electron cryomicroscopy

Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen.^[1] CryoSEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.^[2]^[3]^[4]^[5]

References

^ Terje, Dokland (2006). Techniques in Microscopy for Biomedical Applications. World Scientific Publishing Co Pte Ltd. p. 115. ISBN 978-9812564344.
^ Hickey, Cian D.; Sheehan, Jeremiah J.; Wilkinson, Martin G.; Auty, Mark A. E. (18 February 2015). "Growth and location of bacterial colonies within dairy foods using microscopy techniques: a review" (PDF). Frontiers in Microbiology. 6: 99. doi:10.3389/fmicb.2015.00099. PMC 4332360. PMID 25741328.
^ Lesemann, Dietrich-Eckhardt; Mendgen, Kurt, eds. (1991). Electron Microscopy of Plant Pathogens. Berlin, Heidelberg: Springer Berlin Heidelberg. ISBN 978-3-642-75818-8.
^ Schatten, Heide (2013). Scanning electron microscopy for the life sciences (Online-Ausg. ed.). Cambridge: Cambridge University Press. ISBN 9780521195997.
^ Schatten, Heide; Pawley, James, eds. (2007). Biological low voltage field emission scanning electron microscopy. New York: Springer. ISBN 9780387729725.

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[Dokland_Terje-1] Terje, Dokland (2006). Techniques in Microscopy for Biomedical Applications. World Scientific Publishing Co Pte Ltd. p. 115. ISBN 978-9812564344.

[Hickey2015-2] Hickey, Cian D.; Sheehan, Jeremiah J.; Wilkinson, Martin G.; Auty, Mark A. E. (18 February 2015). "Growth and location of bacterial colonies within dairy foods using microscopy techniques: a review" (PDF). Frontiers in Microbiology. 6: 99. doi:10.3389/fmicb.2015.00099. PMC 4332360. PMID 25741328.

[Lesemann-3] Lesemann, Dietrich-Eckhardt; Mendgen, Kurt, eds. (1991). Electron Microscopy of Plant Pathogens. Berlin, Heidelberg: Springer Berlin Heidelberg. ISBN 978-3-642-75818-8.

[Schatten2013-4] Schatten, Heide (2013). Scanning electron microscopy for the life sciences (Online-Ausg. ed.). Cambridge: Cambridge University Press. ISBN 9780521195997.

[Schatten-5] Schatten, Heide; Pawley, James, eds. (2007). Biological low voltage field emission scanning electron microscopy. New York: Springer. ISBN 9780387729725.

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Scanning electron cryomicroscopy

Scanning electron cryomicroscopy

Scanning electron cryomicroscopy

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