Self-pollination is a form of pollination in which pollen arrives at the stigma of a flower (in flowering plants) or at the ovule (in gymnosperms) of the same plant. The term cross-pollination is used for the opposite case, where pollen from one plant moves to a different plant.

There are two types of self-pollination: in autogamy, pollen is transferred to the stigma of the same flower; in geitonogamy, pollen is transferred from the anther of one flower to the stigma of another flower on the same flowering plant, or from microsporangium to ovule within a single (monoecious) gymnosperm. Some plants have mechanisms that ensure autogamy, such as flowers that do not open (cleistogamy), or stamens that move to come into contact with the stigma.

The term selfing that is often used as a synonym is not limited to self-pollination, but also applies to other types of self-fertilization.

Few plants self-pollinate without the aid of pollen vectors (such as wind or insects). The mechanism is seen most often in some legumes such as peanuts. In another legume, soybeans, the flowers open and remain receptive to insect cross pollination during the day. If this is not accomplished, the flowers self-pollinate as they are closing. Among other plants that can self-pollinate are many kinds of orchids, peas, sunflowers and tridax. Most of the self-pollinating plants have small, relatively inconspicuous flowers that shed pollen directly onto the stigma, sometimes even before the bud opens. Self-pollinated plants expend less energy in the production of pollinator attractants and can grow in areas where the kinds of insects or other animals that might visit them are absent or very scarce—as in the Arctic or at high elevations.

Self-pollination limits the variety of progeny and may depress plant vigor. However, self-pollination can be advantageous, allowing plants to spread beyond the range of suitable pollinators or produce offspring in areas where pollinator populations have been greatly reduced or are naturally variable.

Pollination can also be accomplished by cross-pollination. Cross-pollination is the transfer of pollen, by wind or animals such as insects and birds, from the anther to the stigma of flowers on separate plants.

Both hermaphrodite and monoecious species have the potential for self-pollination leading to self-fertilization unless there is a mechanism to avoid it. 80% of all flowering plants are hermaphroditic, meaning they contain both sexes in the same flower, while 5 percent of plant species are monoecious. The remaining 15% would therefore be dioecious (each plant unisexual). Plants that self-pollinate include several types of orchids, and sunflowers. Dandelions are capable of self-pollination as well as cross-pollination.

There are several advantages for self-pollinating flowers. Firstly, if a given genotype is well-suited for an environment, self-pollination helps to keep this trait stable in the species. Not being dependent on pollinating agents allows self-pollination to occur when bees and wind are nowhere to be found. Self-pollination or cross pollination can be an advantage when the number of flowers is small or they are widely spaced. During self-pollination, the pollen grains are not transmitted from one flower to another. As a result, there is less wastage of pollen. Also, self-pollinating plants do not depend on external carriers. They also cannot make changes in their characters and so the features of a species can be maintained with purity. Self-pollination also helps to preserve parental characters as the gametes from the same flower are evolved. It is not necessary for flowers to produce nectar, scent, or to be colourful in order to attract pollinators.