The Church of Jesus Christ of Latter-day Saints in Florida refers to the Church of Jesus Christ of Latter-day Saints (LDS Church) and its members in Florida. The LDS Church represents about 1% of the population of Florida according to the Pew Research Center 2014 Religious Landscepe Survey. Official membership statistics show the church representing about 0.75% of the general population. Florida has the 8th largest membership population in the United States and the largest membership population east of the Mississippi. The LDS Church is the 6th largest denomination in Florida.

In April 1843, Joseph Smith called William Brown and Daniel Cathcart to serve a mission to Pensacola, but no record exists of them fulfilling the calling. Between April and June 1854, Phineas Young visited the Indian chiefs in Florida and distributed copies of the Book of Mormon.

Missionaries began preaching in Pensacola in January 1895 and started a number of Sunday Schools soon afterwards. The first was in Coe Mills in May 1895. The first branch, known as the Hassell Branch, was created in Jefferson County on May 9, 1897. In September 1897, the Sanderson Branch was organized. George P. Canova, a well-to-do landowner and chairman of the Baker County Commission, became the branch president in January 1898. Five months later, following threats of violence, Canova was killed as he returned home from a church meeting.

In 1906, Charles A. Callis became president of the Florida Conference. That same year, a meetinghouse was dedicated in Jacksonville. Another meetinghouse was completed in Oak Grove in 1907. In 1909, missionaries began working in Miami during the winter months. Three years later, four Latter-day Saint pioneer families from Arizona moved to Florahome, Putnam County and established a Sunday School. In 1914, Julius C. Neubeck of Miami was called on a seven-month mission by Callis and became the first missionary from that city. He then became presiding elder of the church in Miami following his mission.

By 1925, branches or Sunday Schools existed in Florahome, (Putnam County), Jacksonville, Sanderson, Tampa, Miami and in other places throughout the state. In February and March 1925, church president Heber J. Grant visited Jacksonville and held public meetings. Ten years later, the Florida District had 22 branches, and the West Florida District had another 13 branches.

The first stake in Florida and in the South was created in Jacksonville on January 19, 1947, by Callis, who by then was a member of the Quorum of the Twelve. Alvin C. Chace, a grandson of early leader George P. Canova, was called as the first president.

Due to the influx of immigrants Florida received over the past few decades from the Caribbean and other countries, branches and wards were created to accommodate foreign speaking individuals in Florida. The first Spanish-speaking stake in the southeastern United States was organized in Miami. This was followed by the creation of a second Spanish-speaking stake in Hialeah Gardens in 1998.

On October 9, 1994, church president Howard W. Hunter dedicated the Orlando Florida Temple. On January 19, 1997, church president Gordon B. Hinckley addressed more than 5,000 members at a conference in Jacksonville commemorating the stake's 50th anniversary.