Twillingate is a town of 2,121 people located on the Twillingate Islands ("Toulinquet") in Notre Dame Bay, off the north eastern shore of the island of Newfoundland in the province of Newfoundland and Labrador, Canada. The town is about 100 kilometres (62 mi) north of Lewisporte and Gander.

Incorporated on September 30, 1965, the town of Twillingate also incorporates smaller former localities such as Back Harbour, Bayview, Durrell, Gillard's Cove, Jenkins Cove, Manuel's Cove, and Wild Cove. The Twillingate Islands provide an excellent sheltered harbour and easy access to the rich fishing grounds nearby. Twillingate Island is connected to mainland Newfoundland via the Walter B. Elliott causeway as part of Route 340. The town is also one of the oldest ports on the island. It was a historic fishing community, but due to the collapse of the cod fishing industry, its economy now relies more on tourism.

The earliest known people to inhabit the area were the Maritime Archaic, who occupied the area 3,500 years ago in roughly 1500 BC. The Maritime Archaic people were later supplanted by the Beothuk, and possibly the Dorset Inuit, who occupied the area until the arrival of European settlers.

The French had been fishing in the area possibly as early as 1500, but the first European settlers did not arrive until the 17th century. The settlers were mostly made up of fishermen and their families from the West Country in England. The native Beothuk managed to survive until the early 19th century in small numbers near Twillingate and the mouth of the Exploits River.

As the population grew, Twillingate became an important fishing community. It was a busy trade and service centre for Labrador and the northern shore fisheries for more than two centuries. One of the more prominent historical events that occurred in the history of Twillingate was the arrival of a local newspaper – the Twillingate Sun. The Sun served Twillingate and its local area from the 1880s until the 1950s. The Sun was a robust and professional newspaper that not only covered local and provincial news, but also international news as well. Lewisporte based newspaper the Pilot then served the area until going defunct in 2018. Since the Fisheries and Oceans Canada moratorium on fishing northern cod (see Endangered Species Controversies in Canada and Europe) was announced on July 2, 1992, followed shortly after by the collapse of the fishing industry, Twillingate has been forced to look to the tourist industry for income and has become a popular spot for visitors in the summer.

Twillingate is easily accessed by Route 340 from Lewisporte if approaching from western Newfoundland or by Route 330 from Gander if approaching from eastern Newfoundland. The town of Twillingate is approximately 1.5 hours from Gander and about an hour from Lewisporte.

There are several very small fishing communities that can be found on Twillingate Island before reaching the main town, such as Black Duck Cove, Purcell's Harbour, and Little Harbour. The western portion of the island, a series of communities now amalgamated into Bayview is accessible via Bayview Street. The town of Twillingate is about 5 km (3.1 mi) from the Twillingate-New World Island causeway. When in the town, access to the northern island is provided by "Tickle Bridge" and the communities on the southern island can be accessed via the town's main road.

Route 340 runs through Twillingate as Toulinguet Street and Main Street. After turning left at the town's main intersection (Toulinguet and Main), Route 340 runs through northern portion of the town and continues through the surrounding communities on the northern island, Back Harbour, Paradise and Wild Cove, then after a 0.5 km stretch enters the town of Crow Head and continues to the Long Point Lighthouse.