The Aldine Press was the printing office started by Aldus Manutius in 1494 in Venice, from which were issued the celebrated Aldine editions of the classics (Latin and Greek masterpieces, plus a few more modern works). The first book that was dated and printed under his name appeared in 1495.

The Aldine Press is famous in the history of typography, among other things, for the introduction of italics. The press was the first to issue printed books in the small octavo size, similar to that of a modern paperback, and intended for portability and ease of reading. According to Curt F. Bühler, the press issued 132 books during twenty years of activity under Aldus Manutius. After Manutius' death in 1515, the press was continued by his wife Maria and her father Andrea Torresani (Andrea Torresano [Wikidata]), until Manutius' son Paulus (1512–1574) took over. His grandson Aldus Manutius the Younger then ran the firm until his death in 1597. Today, the antique books printed by the Aldine Press in Venice are referred to as Aldines, as are the letterforms and typefaces pioneered by the Aldine Press.

The press enjoyed a monopoly of works printed in Greek in the Republic of Venice, effectively giving it copyright protection. Protection outside the Republic was more problematic, however. The firm maintained an agency in Paris, but its commercial success was affected by many counterfeit editions, produced in Lyon and elsewhere.

Aldus Manutius, the founder of the Aldine Press, was originally a humanist scholar and a teacher. Manutius met Andrea Torresani, who had acquired publishing equipment from the widow of Nicholas Jenson. The ownership of the press was originally split in two, with one half belonging to Pier Francesco Barbarigo, the nephew of Agostino Barbarigo, who was the doge at the time, and the other half belonging to Torresani. Manutius owned one fifth of Torresani's share of the press. Manutius was mainly in charge of the scholarship and editing, leaving financial and operating concerns to Barbarigo and Torresani. In 1496, Manutius established his own location of the press in a building called the Thermae in the Sestiere di San Polo on the campo Sant'Agostin in Venice, today numero civico (house number) 2343 San Polo on the Calle della Chiesa (Alley of the Church), now the location of the restaurant Due Colonne. Though there are two commemorative plaques located on the building numero civico 2311 Rio Terà Secondo, historians regard them to be erroneously placed based on contemporaneous letters addressed to Manutius. The first erroneous plaque had been placed by Abbot don Vincenzo Zenier in 1828.

Manutius lived and worked in the Thermae in order to produce published books from the Aldine Press. This was also the location of the "New Academy", where a group of Manutius' friends, associates, and editors came together to translate Greek and Latin texts. In 1505, Manutius married Maria, the daughter of Torresani. Torresani and Manutius were already business partners, but the marriage combined the two partners' shares in the publishing business. After the marriage, Manutius lived at Torresani's house. Shrinking in popularity, in 1506 the Aldine Press was moved to Torresani's house in the parish of San Paternian. It was later demolished in 1873 and was covered by a bank building in the Venice square, Campo Manin.

The press was started by Manutius due to a combination of his love of classics and the need for preservation of Hellenic studies. During its initial era, the press printed new copies of Plato, Aristotle, and other Greek and Latin classics.

The first edition of Plato's works (known as the Aldine edition) was dedicated to Pope Leo X and included the poem of Musurus and the life of Plato by Diogenes Laertius, which were also included in the first two editions of Plato's works printed in Basel. The two Basel editions were introduced by a Latin preface written by the German humanist Simon Grynaeus, a scholar of Greek, who dedicated the work to the humanist Thomas More.

Manutius also printed dictionaries and grammars to help people interpret the books, used by scholars wanting to learn Greek, who would employ learned Greeks in order to teach them directly. Historian Elizabeth Eisenstein claims that the fall of Constantinople in 1453 had placed under threat the importance and survival of Greek scholarship, but that publications such as those by the Aldine Press secured it once more. Erasmus was one of the scholars learned in Greek with whom the Aldine Press partnered in order to provide accurately translated text. The Aldine Press also expanded into modern languages, mainly Italian and French.