Artificial intelligence detection software aims to determine whether some content (text, image, video or audio) was generated using artificial intelligence (AI). However, this software is often unreliable.

Many AI detection tools have been shown to be unreliable in detecting AI-generated text. In a 2023 study conducted by Weber-Wulff et al., researchers evaluated 14 detection tools including Turnitin and GPTZero and found that "all scored below 80% of accuracy and only 5 over 70%." They also found that these tools tend to have a bias for classifying texts more as human than as AI, and that accuracy of these tools worsens upon paraphrasing.

In AI content detection, a false positive is when human-written work is incorrectly flagged as AI-written. Many AI detection platforms claim to have a minimal level of false positives, with Turnitin claiming a less than 1% false positive rate. However, later research by The Washington Post produced much higher rates of 50%, though they used a smaller sample size. False positives in an academic setting frequently lead to accusations of academic misconduct, which can have serious consequences for a student's academic record. Additionally, studies have shown evidence that many AI detection models are prone to give false positives to work written by those whose first language isn't English and non-autistic people.

In June 2023, Janelle Shane wrote that portions of her book You Look Like a Thing and I Love You were flagged as AI-generated.

A false negative is a failure to identify documents with AI-written text. False negatives often happen as a result of a detection software's sensitivity level or because evasive techniques were used when generating the work to make it sound more human. False negatives are less of a concern academically, since they aren't likely to lead to accusations and ramifications. Notably, Turnitin stated they have a 15% false negative rate.

For text, this is usually done to prevent alleged plagiarism, often by detecting repetition of words as telltale signs that a text was AI-generated (including hallucinations). They are often used by teachers marking their students, usually on an ad hoc basis. Following the release of ChatGPT and similar AI text generative software, many educational establishments have issued policies against the use of AI by students. AI text detection software is also used by those assessing job applicants, as well as online search engines.

Current detectors may sometimes be unreliable and have incorrectly marked work by humans as originating from AI while failing to detect AI-generated work in other instances. MIT Technology Review said that the technology "struggled to pick up ChatGPT-generated text that had been slightly rearranged by humans and obfuscated by a paraphrasing tool". AI text detection software has also been shown to discriminate against non-native speakers of English.

Two students from the University of California, Davis, were referred to the university's Office of Student Success and Judicial Affairs (OSSJA) after their professors scanned their essays with positive results; the first with an AI detector called GPTZero, and the second with an AI detector integration in Turnitin. However, following media coverage, and a thorough investigation, the students were cleared of any wrongdoing.