In music theory, the tritone is defined as a musical interval spanning three adjacent whole tones (six semitones). For instance, the interval from F up to the B above it (in short, F–B) is a tritone as it can be decomposed into the three adjacent whole tones F–G, G–A, and A–B.

Narrowly defined, each of these whole tones must be a step in the scale, so by this definition, within a diatonic scale there is only one tritone for each octave. For instance, the above-mentioned interval F–B is the only tritone formed from the notes of the C major scale. More broadly, a tritone is also commonly defined as any interval with a width of three whole tones (spanning six semitones in the chromatic scale), regardless of scale degrees. According to this definition, a diatonic scale contains two tritones for each octave. For instance, the above-mentioned C major scale contains the tritones F–B (from F to the B above it, also called augmented fourth) and B–F (from B to the F above it, also called diminished fifth, semidiapente, or semitritonus); the latter is decomposed as a semitone B–C, a whole tone C–D, a whole tone D–E, and a semitone E–F, for a total width of three whole tones, but composed as four steps in the scale. In twelve-equal temperament, the tritone divides the octave exactly in half as 6 of 12 semitones or 600 of 1,200 cents.

In classical music, the tritone is a harmonic and melodic dissonance and is important in the study of musical harmony. The tritone can be used to avoid traditional tonality: "Any tendency for a tonality to emerge may be avoided by introducing a note three whole tones distant from the key note of that tonality." The tritone found in the dominant seventh chord can also drive the piece of music towards resolution with its tonic. These various uses exhibit the flexibility, ubiquity, and distinctness of the tritone in music.

The condition of having tritones is called tritonia; that of having no tritones is atritonia^{[citation needed]}. A musical scale or chord containing tritones is called tritonic; one without tritones is atritonic.

Since a chromatic scale is formed by 12 pitches (each a semitone apart from its neighbors), it contains 12 distinct tritones, each starting from a different pitch and spanning six semitones. According to a complex but widely used naming convention, six of them are classified as augmented fourths, and the other six as diminished fifths.

Under that convention, a fourth is an interval encompassing four staff positions, while a fifth encompasses five staff positions (see interval number for more details). The augmented fourth (A4) and diminished fifth (d5) are defined as the intervals produced by widening the perfect fourth and narrowing the perfect fifth by one chromatic semitone. They both span six semitones, and they are the inverse of each other, meaning that their sum is exactly equal to one perfect octave (A4 + d5 = P8). In twelve-tone equal temperament, the most commonly used tuning system, the A4 is equivalent to a d5, as both have the size of exactly half an octave. In most other tuning systems, they are not equivalent, and neither is exactly equal to half an octave.

Any augmented fourth can be decomposed into three whole tones. For instance, the interval F–B is an augmented fourth and can be decomposed into the three adjacent whole tones F–G, G–A, and A–B.

It is not possible to decompose a diminished fifth into three adjacent whole tones. The reason is that a whole tone is a major second, and according to the rule of interval addition explained in Interval (music), the composition of three seconds is always a fourth (for instance, an A4). To obtain a fifth (for instance, a d5), it is necessary to add another second. For instance, using the notes of the C major scale, the diminished fifth B–F can be decomposed into the four adjacent intervals