Enshakushanna (Sumerian: 𒂗𒊮𒊨𒀭𒈾, en-sha3-kush2-an-na; fl. c. 2350 BC), or Enshagsagana, En-shag-kush-ana, Enukduanna, En-Shakansha-Ana, En-šakušuana was a king of Uruk around the mid-3rd millennium BC who is named on the Sumerian King List, which states his reign to have been 60 years. He conquered Hamazi, Akkad, Kish, and Nippur, claiming hegemony over all of Sumer.

He adopted the Sumerian title en ki-en-gi lugal kalam . (𒂗 𒆠𒂗𒄀 𒈗 𒌦), which may be translated as "lord of Sumer and king of all the land" (which possibly implies "en of the region of Uruk and lugal of the region of Ur"), and could correspond to the later title lugal ki-en-gi ki-uri "King of Sumer and Akkad" that eventually came to signify kingship over Mesopotamia as a whole.

Enshakushanna's reign is largely characterized by his military campaigns, the most prominent of which was against Kish and Akshak. His attack on these two cities is attested from a stone bowl at Nippur and reads as follows:

For Enlil, king of all lands,

Enshakushanna, lord of the land of Sumer and king of the nation when the gods commanded him, he sacked Kish (and) captured Enbi-Ishtar, the king of Kish. The leader of Kish and the leader of Akshak, (when) both their cities were destroyed ... (Lacuna) in (?) [..] he returned to them,

but [he] dedicated their statues, their precious metals and lapis lazuli, their timber and treasure, to the god Enlil at [N]ippur.

An inscription on three bowl fragments found at Nippur read:

"For [the god E]nlil, En-šakuš-Ana, dedicated the treasure of sacked Kiš"