The House of Representatives (Dutch: Tweede Kamer der Staten-Generaal [ˈtʋeːdə ˈkaːmər dɛr ˈstaːtə(ŋ) ɣeːnəˈraːl] ^ⓘ, literally "Second Chamber of the States General", or simply the Tweede Kamer) is the lower house of the bicameral parliament of the Netherlands, the States General, the other one being the Senate. It has 150 seats, which are filled through elections using party-list proportional representation. The house is located in the Binnenhof in The Hague; it has temporarily moved to the former building of the Ministry of Foreign Affairs at Bezuidenhoutseweg 67 in The Hague while the Binnenhof is being renovated.

Although the body is officially called the "House of Representatives" in English, it is not a direct translation of its official Dutch name, the "Second Chamber of the States General", "Second Chamber" or more colloquially just the "Chamber". Rather than "representative" (afgevaardigde), a member of the House is referred to as (Tweede) Kamerlid, or "member of the (Second) Chamber".

The House of Representatives is the primary legislative body of the States General, where proposed legislation is discussed and the actions of the cabinet are reviewed. Both the Cabinet and the House of Representatives itself have the right to propose legislation; the House of Representatives discusses it and, if adopted by a majority, sends it on to the Senate. Both individual cabinet ministers and the cabinet as a whole must have parliament’s confidence. Therefore, a minister, or the whole cabinet, must resign if a majority of parliament expresses it no longer has confidence in them. Review of the actions of the cabinet takes the form of formal interrogations in plenary and committee meetings, which may result in motions urging the cabinet to take, or refrain from, certain actions. No individual may be a member of both parliament and cabinet, except in a caretaker cabinet that has not yet been succeeded when a new House is sworn in.

Through functions like the scrutiny and political discussions before meetings of the Council of the European Union, the appointment of EU-rapporteurs, and participation in the Conference of Parliamentary Committees for Union Affairs the House of Representatives also plays a role in EU policy making.

The House of Representatives is also responsible for the first round of selection for judges to the Supreme Court of the Netherlands. It submits a list of three names for every vacant position to the Government. Furthermore, it elects the Dutch Ombudsman and their deputies.

The normal term of the House of Representatives is four years. Elections are called when the government loses the parliament's confidence, the governing coalition breaks down, the term of the House of Representatives expires, or when no governing coalition can be formed.

All citizens over the age of 18 are eligible to vote. Eligible citizens residing in the Netherlands are automatically registered through the municipal population register, while expatriates can permanently register at the municipality of The Hague provided they have a current Dutch passport or national ID. Residents of Aruba, Curaçao, and Sint Maarten can only vote if they have spent at least ten years residing in the Netherlands or work for the Dutch civil service.

Prisoners serving a term of more than one year are not eligible to vote. From 2009 onwards, mentally incapacitated citizens have regained the right to vote.