Following the Civil War, Pemberton re-established himself as a pharmacist, opening a drugstore in Columbus, Georgia. This business served as a base for his pharmaceutical experiments and formulations.

Pemberton dedicated his time to creating various medicinal and tonic formulations. He developed a range of products, including medicinal extracts, elixirs, and tonics, sold through his pharmacy. He was always looking for innovative remedies.

Pemberton relocated to Atlanta, Georgia, recognizing the city's growing economic opportunities. This move proved pivotal in his career, leading to the development and marketing of Coca-Cola.

Pemberton created 'Pemberton's French Wine Coca,' a nerve tonic and patent medicine containing coca leaves and kola nuts mixed with wine. This product was marketed as a remedy for nervous affections, headaches, and other ailments, and predated his more famous creation.