There are various intersections of the LGBTQ community and Wikipedia. LGBTQ people who edit the online encyclopedia often face cyberbullying and other types of harassment. Wikipedia content about LGBTQ individuals is often vandalized, but various Wikipedia user groups, WikiProjects, and the Wikimedia Foundation endorse campaigns to promote inclusion on Wikipedia. Availability of Wikipedia's LGBTQ content, in countries that otherwise suppress information about LGBTQ issues, has been praised.

In 2011, the Wikimedia Foundation (WMF) made it a strategic goal to recruit more women, people of color, and other underrepresented individuals as editors, including LGBTQ people.

In 2019, Rachel Wexelbaum, an associate professor at St. Cloud State University in Minnesota, United States, wrote, "For LGBTIQ+ people and those searching for LGBTIQ+ information, Wikipedia has proven invaluable in countries where LGBTIQ+ publications, media, or visibility may be criminalized or cut short due to AIDS NGOs leaving those countries." It can also be valuable for those in communities where this information is socially marginalized; a notable example is the experience of transgender author and activist Abby Stein, who discovered the idea of being transgender on the Hebrew Wikipedia. Wikipedia is often consulted by LGBTQ youth seeking information on sexual health, as Wikipedia's coverage of health-related topics is backed by numerous medical journals. Some Wikipedia editors, however, have reported struggles with encouraging LGBTQ health organizations to participate in contributing LGBTQ-specific health information to Wikipedia.

In some cases, particular language editions of Wikipedia have slanted toward anti-LGBTQ content. The Croatian Wikipedia has been criticized for advancing homophobic propaganda and for other reasons. In addition, the only active administrator of Amharic Wikipedia, at one point, enforced the Ethiopian government's anti-LGBTQ laws on the wiki. According to Business Insider, an anonymous editor using an IP address coming from the United States House of Representatives, who claimed to be a Capitol Hill staffer, made a series of edits about the transgender community, including some that were critical of transgender individuals.

The English Wikipedia's style guidelines on identity state editors should describe transgender and non-binary subjects using their preferred name and pronouns corresponding to their most recently stated gender identity. However, such articles are frequently targeted with vandalism, misgendering or deadnaming their subjects. In August 2008, the article about Ina Fried, a transgender journalist for CNET, was caught in an edit war over which pronouns to use for her. She stated that Wikipedia did not have a stylebook on gender, unlike the Associated Press Stylebook, and said that while she found it "somewhat confusing" to see the gender changes on her page, she "found the debate interesting." She later added that it was a "reasonable compromise" to remove all pronouns in her biography entry.

After Chelsea Manning came out in August 2013, editors debated the title of the article about her. At the time, Slate praised actions by Wikipedia editors, saying that Manning's article was rewritten quickly and with "remarkably little controversy". However, in October 2013, The Guardian noted that the English Wikipedia's Arbitration Committee had "banned a number of editors from working on articles related to transgender topics or individuals," noting that while some were banned for "making transphobic comments about Manning", others received the same punishment "for pointing out the bigotry". Two of the restricted editors had insisted on referring to Manning as a man, while another editor who alleged the existence of "a 'consensus' of virulently transphobic" Wikipedia editors was sanctioned for a "battleground approach" by the committee.

Following Caitlyn Jenner's gender transition in 2015, Kat George of Bustle wrote, "We can start learning about the proper use of gender pronouns, with Caitlyn Jenner's Wikipedia article as a perfect example of the correct before and after language we should be employing." The name and pronouns to use for Gloria Hemingway were a matter of discussion for over 15 years. In February 2022, after a week of debate, votes were evenly split between using Gloria and "she/her" pronouns, or continuing to use her birth name. An editor closed the discussion in favor of renaming; the decision was appealed but upheld by an administrator.

Wikipedia editors experience harassment, and in one case, a transgender editor was publicly deadnamed. The WMF has expressed concern over situations where transgender editors could be repelled from Wikipedia due to online abuse. BBC News said in 2020, "Many, particularly women and members of the LGBTQ community, have complained of abuse and harassment from other editors." Editors can report harassment to administrators via email or notice boards, which can cause harassers to be barred from editing.