Microneurography is a neurophysiological method employed to visualize and record the traffic of nerve impulses that are conducted in peripheral nerves of waking human subjects. It can also be used in animal recordings. The method has been successfully employed to reveal functional properties of a number of neural systems, e.g. sensory systems related to touch, pain, and muscle sense as well as sympathetic activity controlling the constriction state of blood vessels. To study nerve impulses of an identified nerve, a fine tungsten needle microelectrode is inserted into the nerve and connected to a high input impedance differential amplifier. The exact position of the electrode tip within the nerve is then adjusted in minute steps until the electrode discriminates nerve impulses of interest. A unique feature and a significant strength of the microneurography method is that subjects are fully awake and able to cooperate in tests requiring mental attention, while impulses in a representative nerve fibre or set of nerve fibres are recorded, e.g. when cutaneous sense organs are stimulated or subjects perform voluntary precision movements.

Before the microneurography technique was developed in the late 1960s, impulses in peripheral nerves had been recorded in animal experiments alone using a technique that involved dissection and splitting the nerve. This approach is not tolerable for general use in humans although it has been pursued in one single study. Actually, the concern of nerve damage was a major obstacle for the development of microneurography because the approach of inserting a needle electrode in a human nerve was generally regarded as potentially dangerous and involving substantial risk of permanent nerve damage. The two Swedish scientists who developed the microneuropgraphy technique (Hagbarth and Vallbo) handled the medical-ethical concern by performing a large series of experiments on their own nerves during a period of about 2 years while carefully checking for nerve damage. Working at the Department of Clinical Neurophysiology, Academic Hospital, Uppsala, they collected data resulting in the first papers representing three areas to become major fields of microneurography, i.e. afference from intra-muscular sense organs during voluntary contractions, response of cutaneous sense organs related to touch stimuli, and efferent sympathetic activity controlling the constriction of human blood vessels. The microneurography approach of Hagbarth and Vallbo based on epoxy resin coated tungsten microelectrodes is now generally accepted whereas an alternative attempt using glass coated platina-iridium electrodes had obviously limited success as it yielded a single short note alone.

Nerve fibers (axons) of various kinds are more or less randomly mixed in most nerves. This is true for fibers of different functions as well as fibers of different sizes. Basically fiber diameter is closely related to function, e.g. the cutaneous pain system is dependent on small nerve fibers whereas discriminative touch is dependent on large fibers. With regard to fiber diameter there are two main categories: myelinated A-fibers are large and conduct impulses at high or moderate speed (5–75 m/s) while unmyelinated C-fibers are small and conduct impulses at low speed (around 1 m/s). In microneurography recordings, A- and C-fiber impulses differ in shape and polarity of the main upstroke of the action potential. Because fibers are mixed in most nerves, it is usually essential to record from an individual nerve fiber at a time to explore the properties of a functional system, although multi-unit recording has been very rewarding in studies of sympathetic efferent activity. An individual nerve consists of a number of fascicles, i.e. bundles of nerve fibers enclosed within a connective tissue sheath containing the different nerve fibers. Therefore, the tip of the microelectrode needs to be not only intraneural but also intrafascicular for a recording to be possible.

Microneurography is based on tungsten needle microelectrodes which are inserted through the skin and into a nerve. Anaesthetics are not required because the procedure induces only minimal discomfort. The tungsten microelectrodes have a shaft diameter of 100-200 μm, a tip diameter of 1-5 μm, and they are insulated to the tip with an epoxy resin. Electrode impedance varies between 0.3 and 5 MΩ at 1 kHz as measured initially. However, the impedance tends to decrease during experiment and is usually below 1 MΩ while impulses are recorded. Nerve discharges are determined by voltage differences between the intra-neural electrode and a reference needle electrode in the vicinity. The 2 electrodes are connected to a differential amplifier with a high input impedance and an appropriate band-pass filtering, often 500 to 5000 Hz. Signals are monitored on a computer screen and stored on a hard disc for off-line analysis. Any peripheral nerve that can be reached may be a target for microneurography recordings, typically in the arm or leg, although recording from facial nerves and the vagus nerve have also been achieved. In order to locate deep nerves, electrical stimulation through a needle electrode or ultrasonic monitoring is often used. This is rarely needed when recording from cutaneous superficial nerves, such as the superficial peroneal or superficial radial nerves, that can easily be located visually and by palpation. When electrical localization is needed, weak electrical shocks are delivered either through the recording electrode or through a separate stimulation needle while adjusting the electrode tip until a neural response is observed, either a muscle twitch or a cutaneous sensation reported by the subject. In ultrasonic monitoring, a linear, high frequency ultrasound probe is used. The microelectrode is then inserted 1–2 cm from the probe, ideally in a 90° angle to the ultrasonic beam. This generates the best wave reflection and image. Ultrasonic approach accurately locates the depth of the nerve and identifies surrounding anatomical structures of interest, such as blood vessels and bony structures, which may affect the placement of a microelectrode. A particular advantage is that the ultrasonic approach visualizes the electrode and the nerve at the same time, thereby facilitating electrode manipulation to reach the nerve. Once the electrode tip is in the nerve, small adjustments are required, first, to penetrate the sheath of an individual fascicle and, second, to take the tip close to the nerve fibers of the kind you are interested to explore, be it multi-unit sympathetic activity or single unit activity of either a myelinated afferent or a small unmyelinated fibres.

Recording of single afferent impulses from C-fibers was greatly improved by the development of the so-called 'marking technique'. This technique is based on a unique property of many kinds of C-fibres, i.e. a decrease of conduction velocity in the wake of preceding impulses. By combining repetitive electrical stimulation and physical stimulation, e.g. mechanical or thermal stimuli, units responding to the physical stimulation will display sudden slowing of their conduction velocity which can be easily visualized in raster plots of latency. This allows identification and characterization of nerve fiber responsiveness to natural stimulation. The marking technique is very efficient as it allows simultaneous recordings of several fibers. However, it generates only semi-quantitative information about unitary activity, whereas recordings of impulse trains allow more comprehensive description of functional properties of sense organs.^{[citation needed]}

The microneurography electrode may be used not only for recording of nerve impulses but for stimulation of individual fibers as well. An interesting application is to combine successive recording and stimulation of the same afferent. Once the functional properties of an afferent have been defined, e.g. with regard to sensitivity, receptive field structure, and adaptation, the electrode may be reconnected to a stimulator to give trains of electrical pulses of controlled strength, rate, and duration. It has been found that the percept elicited from a single tactile afferent in the glabrous skin of the hand, may be remarkably detailed and closely matching the properties of the afferent, indicating a high degree of specificity. Although this approach to bridge the gap between biophysical events in a single afferent and mental phenomena within the mind is simple and straight forward in principle it is demanding in practice for a number of reasons. Micro-stimulation has also been used to characterize individual motor units with regard to contraction properties.^{[citation needed]}

Microneurography recordings have elucidated the organization as well as normal and pathological function of a fair number of neural systems in human. Recently, the technique has also been used in clinical situations for diagnostic purposes to clarify the condition of the individual patient. Three main groups of neural systems have been explored, i.e. proprioception, cutaneous sensibility, and sympathetic efferent activity.

Information from a variety of sense organs provides information about joint positions and movements. The most elaborate proprioceptive sense organ is the muscle spindle. It is unique because its functional state is continually controlled from the brain through the fusimotor system. Recordings from muscle spindle afferents indicate that the fusimotor system remains largely passive when the parent muscle is relaxed whereas is it regularly activated in voluntary contractions and more so the stronger the contraction. Thus microneurography suggests a parallelism between the two motor systems, i.e. the skeletomotor system controlling the ordinary muscle fibers and the fusimotor system. This seems to hold at least for weak contractions and small movements which have been explored so far. In contrast, more independent fusimotor activity has been reported in animal experiments, mainly cat hind limb, where larger movements are allowed. Thanks to fusimotor activation, the afferent signal from muscle spindles remains efficient in monitoring large changes of muscle length without turning silent during muscle shortening. On the other hand, very small intramuscular events are monitored as well, thanks to the extreme sensitivity of the sense organ. An example is the small pulsatile component of the muscle contraction which is due to a periodic fluctuation at 8–10 Hz of the motor command. These small variations are insentient but readily monitored by the population of spindle afferents. They are akin to the tremor we may experience when emotionally excited. The functional significance of the insentient spindle response to faint intramuscular events remains to be assessed. However, it seems likely that detailed information on large as well as small mechanical events in the muscles is essential for neural systems in the brain to produce appropriate commands for dexterous movements.