Mr. Freeze's original comic book appearances established his basic backstory as a scientist transformed by a cryogenic accident. However, his motivations and characterization have evolved over time.

Batman: The Animated Series redefined Mr. Freeze as a more sympathetic and tragic villain, emphasizing his love for Nora and his desperation to save her life. This portrayal has become the definitive version of the character for many fans.

Mr. Freeze has been portrayed in live-action films with varying degrees of success. Some adaptations have remained faithful to his tragic backstory, while others have focused on his villainous aspects or exaggerated his comedic potential.

Mr. Freeze has appeared in numerous video games, television series, and other media adaptations, each offering a unique interpretation of his character and story. These adaptations often explore different aspects of his personality, motivations, and relationships.