Muisca religion describes the religion of the Muisca who inhabited the central highlands of the Colombian Andes before the Spanish conquest of the Muisca. The Muisca formed a confederation of holy rulers and had a variety of deities, temples and rituals incorporated in their culture. Supreme being of the Muisca was Chiminigagua who created light and the Earth. He was not directly honoured, yet that was done through Chía, goddess of the Moon, and her husband Sué, god of the Sun. The representation of the two main celestial bodies as husband and wife showed the complementary character of man and woman and the sacred status of marriage.

The Muisca worshipped their gods at sacred sites, both natural, such as Lake Guatavita, the Siecha Lakes and Lake Tota and constructed; the Sun and Moon Temples in respectively Suamox (the "Rome" or "Mecca" of the Muisca) and Chía, City of the Moon. During these rituals the priests, obgues, performed sacrifices, sometimes human in character. The last public religious ceremony of the Muisca was performed in Ubaque on December 27, 1563.

Knowledge about the Muisca religion was brought to Europe by conquistador Gonzalo Jiménez de Quesada and soldier Juan de Castellanos in the 16th century and by bishop Lucas Fernández de Piedrahita and friar Pedro Simón in the 17th century. Modern Muisca scholars who wrote about the religion of the inhabitants of the Altiplano Cundiboyacense are Javier Ocampo López and Eduard Londoño.

The Muisca were deeply religious people and their rulers had a double role both as political and as religious leaders. The people fasted and also consumed coca, tobacco and yopo with their rituals. Yopo was extracted from Anadenanthera trees, growing in the Llanos Orientales, to the east of the Muisca territories. The psychoactive seeds of the tree were traded with the Achagua, Guayupe and Tegua and grinded and inhaled using a hollow bird bone or a spoon. The plates from which the yopo was inhaled were made of gold and tumbaga and well elaborated and decorated. Many of them have been found and are on display in the Museo del Oro. Coca was used in rituals of predictions and to heal diseases. The coca was combined with cal to increase the efficiency of the substance. The cal was saved in poporos, often made of gold or tumbaga.

A variety of deities have been described by the chroniclers.

Chiminigagua was the creator god of the Muisca who made the light and the Earth. At the beginning of time it was all dark and Chiminigagua sent two large black birds into the skies. From their beaks the light was created and the cosmos illuminated.

Chía was the goddess of the Moon and one of the two gods through which Chiminigagua was honoured. She represented fertility of the Earth and of the people. Chía was married to Súe.

Súe was the god of the Sun, important for the agriculture of the Muisca. He and his wife Chía followed each other across the skies, forming the perfect couple in conjunction at New Moon and during solar and lunar eclipses.