The four classical muscles of mastication elevate the mandible (closing the jaw) and move it forward/backward and laterally, facilitating biting and chewing. Other muscles are responsible for opening the jaw, namely the geniohyoid, mylohyoid, and digastric muscles (the lateral pterygoid may play a role).

The muscles are:

In humans, the mandible, or lower jaw, is connected to the temporal bone of the skull via the temporomandibular joint. This is an extremely complex joint which permits movement in all planes. The muscles of mastication originate on the skull and insert into the mandible, thereby allowing for jaw movements during contraction.

Each of these primary muscles of mastication is paired, with each side of the mandible possessing one of the four.

Unlike most of the other facial muscles, which are innervated by the facial nerve (or CN VII), the muscles of mastication are innervated by the trigeminal nerve (or CN V). More specifically, they are innervated by the mandibular branch, or V₃. The mandibular nerve is both sensory and motor.

Embryologically, the muscles of mastication are all derived from the first pharyngeal arch.

The muscles of facial expression, on the other hand, are derived from the second pharyngeal arch.

The mandible is the only bone that moves during mastication and other activities, such as talking.