Ottoman Syria (Arabic: سوريا العثمانية) is a historiographical term used to describe the group of divisions of the Ottoman Empire within the region of the Levant, usually defined as being east of the Mediterranean Sea, west of the Euphrates River, north of the Arabian Desert and south of the Taurus Mountains.

Ottoman Syria was organized by the Ottomans upon conquest from the Mamluk Sultanate in the early 16th century as a single eyalet (province) of the Damascus Eyalet. In 1534, the Aleppo Eyalet was split into a separate administration. The Tripoli Eyalet was formed out of Damascus province in 1579 and later the Adana Eyalet was split from Aleppo. In 1660, the Eyalet of Safed was established and shortly afterwards renamed the Sidon Eyalet; in 1667, the Mount Lebanon Emirate was given special autonomous status within the Sidon province, but was abolished in 1841 and reconfigured in 1861 as the Mount Lebanon Mutasarrifate. The Syrian eyalets were later transformed into the Syria Vilayet, the Aleppo Vilayet and the Beirut Vilayet, following the 1864 Tanzimat reforms. Finally, in 1872, the Mutasarrifate of Jerusalem was split from the Syria Vilayet into an autonomous administration with a special status.

Before 1516, Syria was part of the Mamluk Empire centered in Lower Egypt. The Ottoman Sultan Selim I conquered Syria in 1516 after defeating the Mamlukes at the Battle of Marj Dabiq near Aleppo in northern Syria. Selim carried on his victorious campaign against the Mamlukes and conquered Egypt in 1517 following the Battle of Ridanieh, bringing an end to the Mamluk Sultanate.

When he first seized Syria in 1516, Selim I kept the administrative subdivisions of the Mamluk period unchanged. After he came back from Egypt in July 1517, he reorganized Syria into one large province or eyalet named Şam (Arabic/Turkish for "Syria"). The eyalet was subdivided into several districts or sanjaks.

In 1549, Syria was reorganized into two eyalets. The northern Sanjak of Aleppo became the center of the new Eyalet of Aleppo. At this time, the two Syrian Eyalets were subdivided as follows:

In 1579, the Eyalet of Tripoli was established under the name of Tripoli of Syria (Turkish: Trablusşam; Arabic: طرابلس الشام). At this time, the eyalets became as follows:

The Eyalet of Aleppo included the Sanjaks of Aleppo, Adana, Marash, Aintab, and Urfa.

The Eyalet of Tripoli included the Sanjaks of Tripoli, Latakia, Hama and Homs.