Pirlimycin, sold under the brand name Pirsue, is used in the treatment of mastitis in cattle. It is used as the salt pirlimycin hydrochloride and it belongs to the lincosamide class of antimicrobials.

Pirlimycin is active against Gram-positive bacteria, specifically Staphylococcus aureus and coagulase negative species of Staphylococcus and Streptococcus. It has no activity against Gram-negative bacteria.

It is bacteriostatic and acts by inhibiting bacterial protein synthesis via binding with the 50S subunit of the ribosome.^{[citation needed]}