Samuel is a figure who, in the narratives of the Hebrew Bible, plays a key role in the transition from the biblical judges to the United Kingdom of Israel under Saul, and again in the monarchy's transition from Saul to David. He is venerated as a prophet in Judaism, Christianity, and Islam. In addition to his role in the Bible, Samuel is mentioned in Jewish rabbinical literature, in the Christian New Testament, and in the second chapter of the Quran (although the text does not mention him by name). He is also treated in the fifth through seventh books of Antiquities of the Jews, written by the Jewish scholar Josephus in the first century. He is first called "the Seer" in 1 Samuel 9:9.

Samuel's mother was Hannah and his father was Elkanah. Elkanah lived at Ramathaim in the district of Zuph. His genealogy is also found in a pedigree of the Kohathites (1 Chronicles 6:3–15) and in that of Heman the Ezrahite, apparently his grandson (1 Chronicles 6:18–33).

According to the genealogical tables in Chronicles, Elkanah was a Levite—a fact not mentioned in the books of Samuel. The fact that Elkanah, a Levite, was denominated an Ephraimite is analogous to the designation of a Levite belonging to Judah (Judges 17:7, for example).

According to 1 Samuel 1:1–28, Elkanah had two wives, Peninnah and Hannah. Peninnah had children; Hannah did not. Nonetheless, Elkanah favored Hannah. Jealous, Peninnah reproached Hannah for her lack of children, causing Hannah much heartache. Elkanah was a devout man and would periodically take his family on pilgrimage to the holy site of Shiloh.

On one occasion, Hannah went to the sanctuary and prayed for a child. In tears, she vowed that if she were granted a child, she would dedicate him to God as a nazirite. Eli, who was sitting at the foot of the doorpost in the sanctuary at Shiloh, saw her apparently mumbling to herself and thought she was drunk, but was soon assured of both her motivation and sobriety. Eli was the priest of Shiloh, and one of the last Israelite Judges before the rule of kings in ancient Israel. He had assumed the leadership after Samson's death. Eli blessed her and she returned home. Subsequently, Hannah became pregnant, later giving birth to Samuel, and praised God for his mercy and faithfulness.

After the child was weaned, she left him in Eli's care, and from time to time she would come to visit her son.

According to 1 Samuel 1:20, Hannah named Samuel to commemorate her prayer to God for a child. "... [She] called his name Samuel, saying, Because I have asked him of the Lord" (KJV). From its appearance, the name Samuel (Hebrew: שְׁמוּאֵל Šəmūʾēl, Tiberian: Šămūʾēl) appears to be constructed from the Hebrew Śāmū (שָׂמוּ) + ʾĒl, meaning "God has set" or "God has placed". This meaning relating to the idea of God setting/placing a child in the womb, alongside Hannah dedicating Samuel as a Nazirite to God. The Hebrew śāmū is also related to the Akkadian šâmū (𒊮𒈬), which shares the same meaning. From the explanation given in 1 Samuel 1:20, however, it would seem to come from a contraction of the Hebrew שְׁאִלְתִּיו מֵאֵל (Modern: Šəʾīltīv mēʾĒl, Tiberian: Šĭʾīltīw mēʾĒl), meaning "I have asked/borrowed him from God". Further shortened to שָׁאוּל מֵאֵל (Šāʾūl mēʾĒl, "asked/borrowed from God"), then finally contracted to שְׁמוּאֵל (Šəmūʾēl/Šămūʾēl). This meaning also relating to Hannah dedicating Samuel as a Nazirite to God as well.

Samuel worked under Eli in the service of the shrine at Shiloh. One night, Samuel heard a voice calling his name. According to the first-century Jewish historian Josephus, Samuel was 12 years old. Samuel initially assumed it was coming from Eli and went to Eli to ask what he wanted. Eli, however, sent Samuel back to sleep. After this happened three times, Eli realised that the voice was the Lord's, and instructed Samuel on how to answer: