WTXL-TV (channel 27) is a television station in Tallahassee, Florida, United States, affiliated with ABC and owned by the E. W. Scripps Company. The station's studios are located on Commerce Boulevard in Midway, Florida, and its transmitter is located near unincorporated Fincher (in northwestern Jefferson County, Florida), along the Georgia state line.

The station debuted on September 16, 1976, as WECA-TV, owned by local businessman Evans Craig Allen. In the early years, its slogan was "We Can Do It!" which was a play on the call letters. The station was the second commercial station to sign-on in the market. Prior to channel 27's arrival, CBS affiliate WCTV had been the sole commercial outlet in the area and carried ABC in off-hours.

Tallahassee had a very long wait for a second station, even though it had been big enough to support at least two stations by the late 1950s and three by the 1960s. However, the Tallahassee market is one of the largest geographic markets east of the Mississippi, stretching across most of the central Florida Panhandle and much of Southwestern Georgia. Only two VHF licenses were allocated to the Tallahassee area—WCTV on channel 6, and non-commercial educational (later PBS member) WFSU-TV on channel 11. UHF stations do not carry well across large areas, making potential station owners skittish about applying for the available UHF channels in the area. By the 1970s, however, cable had gained enough penetration to make a UHF station viable.

Its original studios were on Thomasville Road (US 319/SR 61) in Tallahassee, and it aired an analog signal on UHF channel 27 from a transmitter located at the facilities. By its fourth year of broadcasting, WECA used the slogan "Up & Coming".

In 1984, Allen sold the station to Tallahassee 27 Limited Partnership, led by former Senator Joseph Tydings and former Representative Louis Frey, Jr. The call letters were the changed to the current WTXL-TV on June 25. The station's history page claims the new owners took over in 1985, but according to Federal Communications Commission (FCC) files, the call letters were switched in 1984.

In September 1998, WTXL established a cable-only affiliate of The WB. Known as "WBXT" and originally on-air as "WB 28" (based on the cable channel location), it was part of the national WB 100+ service. Since it was available exclusively on cable, the call sign was fictional in nature and thus not officially recognized by the FCC. WTXL provided local advertisements and performed promotional duties for "WBXT". On April 1, 2005, UPN affiliates WFXU/WTLF switched to The WB through The WB 100+. UPN promptly signed with WCTV which launched a new second digital subchannel to carry the network. As a result, the "WBXT" operation was shut down.

In 2001, Media Ventures Management (then owner of WTXL) entered into an operational outsourcing agreement with rival NBC affiliate WTWC-TV (channel 40, owned by the Sinclair Broadcast Group) which resulted in that station controlling WTXL and "WBXT". On March 17, 2002, this outlet merged all of its operations into WTWC's studios (on Deerlake South in unincorporated Leon County, Florida northwest of Bradfordville). The agreement between WTXL and WTWC was the first of its kind in the United States and was something similar to arrangements known today as local marketing and shared services agreements. The Southern Broadcast Corporation (which it would be renamed Calkins Media) acquired WTXL's license on November 30, 2005, but allowed the outsourcing agreement to continue. On February 20, 2006, the partnership between the two stations was dissolved when the Southern Broadcast Corporation gave notice to terminate the agreement with Sinclair. As a result, WTXL moved out of the WTWC building.

After leaving WTWC's facilities, WTXL temporarily rented studio space from WFSU-TV on the campus of Florida State University. On June 20, 2006, this station broke ground on new studios in a commercial park in nearby Midway. The station fully moved into the new facility in August 2007. WTXL has been digital-only since February 17, 2009. Between January 1992 and April 26, 2011, WTXL served as ABC's affiliate of record for Southwest Georgia, as that area did not have an affiliate of its own after WVGA ceased operations due to financial issues. The following day, WALB added ABC to its second digital subchannel.