Atomic absorption spectroscopy

In order to analyze a sample for its atomic constituents, it has to be atomized. The atomizers most commonly used nowadays are flames and electrothermal (graphite tube) atomizers. The atoms should then be irradiated by optical radiation, and the radiation source could be an element-specific line radiation source or a continuum radiation source. The radiation then passes through a monochromator in order to separate the element-specific radiation from any other radiation emitted by the radiation source, which is finally measured by a detector.

Most commonly used are spectroscopic flames and electrothermal atomizers. Other atomizers, such as glow-discharge atomization, hydride atomization, or cold-vapor atomization, might be used for special purposes.

The oldest and most commonly used atomizers in AAS are flames, principally the air-acetylene (C₂H₂) flame with a temperature of about 2300 °C, and the nitrous oxide (N₂O)^[5]-acetylene flame with a temperature of about 2700 °C. The latter flame offers a more reducing environment, ideally suited for analytes with a high affinity to oxygen.

Liquid or dissolved samples are typically used with flame atomizers. The sample solution is aspirated by a pneumatic analytical nebulizer, transformed into an aerosol, which is introduced into a spray chamber, where it is mixed with the flame gases and conditioned so that only the finest droplets (< 10 μm) enter the flame. This conditioning reduces interference, but causes only about 5% of the solution to reach the flame.

On top of the spray chamber is a burner head that produces a flame that is laterally long (usually 5–10 cm) and only a few mm deep. The radiation beam passes through the long axis, and the flame gas flow-rates may be adjusted to produce the highest concentration of free atoms. The burner height may also be adjusted so that the radiation beam passes through the zone of highest atom cloud density in the flame, resulting in the highest sensitivity.

The flame processes include:

• desolvation (drying), in which the solvent is evaporated leaving the dry sample nano-particles
• vaporization, in which the solid particles are converted into gaseous molecules
• atomization in which the molecules are dissociated into free atoms, and
• ionization, where (depending on the ionization potential of the analyte atoms and the energy of the flame) atoms may be partially converted to gaseous ions

Each of these stages includes the risk of interference if the degree of phase transfer is different for the analyte in the calibration standard and in the sample. Ionization is usually undesirable, as it reduces the number of atoms that are available for measurement, i.e., the sensitivity.

In flame AAS, a steady-state signal is generated while the sample is aspirated. This technique is typically used for determinations in the mg/L range and may be extended down to a few μg/L for some elements.

Electrothermal AAS (ET AAS) using graphite tube atomizers was pioneered by Boris V. L'vov at the Saint Petersburg Polytechnical Institute, Russia,^[6] since the late 1950s, and investigated in parallel by Hans Massmann at the Institute of Spectrochemistry and Applied Spectroscopy (ISAS) in Dortmund, Germany.^[7]

Although a wide variety of graphite tube designs have been used over the years, typical dimensions are 20–25 mm in length and 5–6 mm inner diameter. With this technique liquid/dissolved, solid, and gaseous samples may be analyzed directly. A measured volume (typically 10–50 μL) or a weighed mass (typically around 1 mg) of a solid sample is introduced into the graphite tube and subject to a temperature program. This typically consists of stages of drying – the solvent is evaporated; pyrolysis – the majority of the matrix constituents are removed; atomization – the analyte element is released to the gaseous phase; and cleaning – residues left in the graphite tube are removed at high temperature.^[8]

The graphite tubes are heated via their ohmic resistance using a low-voltage high-current power supply; the temperature in the individual stages can be controlled very closely, and temperature ramps between the individual stages facilitate the separation of sample components. Tubes may be heated transversely or longitudinally, with the former method having a more homogeneous temperature distribution. The so-called stabilized temperature platform furnace (STPF), proposed by Walter Slavin, based on research of Boris L'vov, makes ET AAS essentially free from interference.^[9] The major components of this concept are atomization of the sample from a graphite platform inserted into the graphite tube (L'vov platform) instead of from the tube wall in order to delay atomization until the gas phase in the atomizer has reached a stable temperature; use of a chemical modifier to stabilize the analyte to a pyrolysis temperature that is sufficient to remove the majority of the matrix components; and integration of the absorbance over the time of the transient absorption signal instead of using peak height absorbance for quantification.

In ET AAS, a transient signal is generated, the area of which is directly proportional to the mass of analyte (not its concentration) introduced into the graphite tube. This technique has the advantage that any kind of sample, solid, liquid, or gaseous, can be analyzed directly. Its sensitivity is 2–3 orders of magnitude higher than that of flame AAS, so that determinations in the low μg L⁻¹ range (for a typical sample volume of 20 μL) and ng g⁻¹ range (for a typical sample mass of 1 mg) can be carried out. It has a very high degree of freedom from interferences, so that ET AAS may be considered the most robust technique available for the determination of trace elements in complex matrices.^{[citation needed]}

While flame and electrothermal vaporizers are the most common atomization techniques, several other methods are available for specialized use.^[10][11]

A glow-discharge device (GD) is a versatile source, as it can simultaneously introduce and atomize the sample. The glow discharge occurs in a low-pressure argon gas atmosphere between 1 and 10 torr. In this atmosphere is a pair of electrodes applying a DC voltage of 250 to 1000 V to break down the argon gas into positively charged ions and electrons. These ions, under the influence of the electric field, are accelerated into the cathode surface containing the sample, bombarding the sample and causing neutral sample atom ejection through sputtering. The atomic vapor produced by this discharge is composed of ions, ground state atoms, and a fraction of excited atoms. When the excited atoms relax back into their ground state, a low-intensity glow is emitted, giving the technique its name.

The requirement for samples of glow discharge atomizers is that they are electrical conductors. Consequently, atomizers are most commonly used in the analysis of metals and other conducting samples. However, with proper modifications, it can be used to analyze liquid samples as well as nonconducting materials by mixing them with a conductor (e.g. graphite).

Hydride generation techniques use specialized solutions of specific elements. The technique provides a means of introducing samples containing arsenic, antimony, selenium, bismuth, and lead into an atomizer in the gas phase. With these elements, hydride atomization enhances detection limits by a factor of 10 to 100 compared to alternative methods. Hydride generation occurs by adding an acidified aqueous solution of the sample to a 1% aqueous solution of sodium borohydride, all of which is contained in a glass vessel. The volatile hydride generated by the reaction that occurs is swept into the atomization chamber by an inert gas, where it undergoes decomposition. This process forms an atomized form of the analyte, which can then be measured by absorption or emission spectrometry.

Cold-vapor atomization is a method limited to the determination of mercury due to it being the only metallic element with a high vapor pressure at ambient temperature.^[12] Because of this, it is important for determining organic mercury compounds in samples and their distribution in the environment. The method begins by converting mercury into Hg²⁺ by oxidation from nitric and sulfuric acids, followed by a reduction of Hg²⁺ with tin(II) chloride. The mercury is then swept into a long-pass absorption tube by bubbling a stream of inert gas through the reaction mixture. The concentration is determined by measuring the absorbance of this gas at 253.7 nm. Detection limits for this technique are in the parts-per-billion range, making it an excellent mercury detection method.

The line source AAS (LS AAS) and continuum source AAS (CS AAS) must be distinguished. In classical LS AAS, as proposed by Alan Walsh,^[13] the high spectral resolution required for AAS measurements is provided by the radiation source itself that emits the spectrum of the analyte in the form of lines that are narrower than the absorption lines. Continuum sources, such as deuterium lamps, are only used for background correction. The advantage of this technique is that only a medium-resolution monochromator is necessary for measuring AAS; however, it has the disadvantage that a separate lamp is usually required for each element to be determined. In CS AAS, in contrast, a single lamp, emitting a continuous spectrum over the entire spectral range of interest is used for all elements. Obviously, a high-resolution monochromator is required for this technique, as will be discussed.

Hollow cathode lamps (HCL) are the most common radiation source in LS AAS.^{[citation needed]} Inside the sealed lamp, filled with argon or neon gas at low pressure, is a cylindrical metal cathode containing the element of interest and an anode. A high voltage is applied across the anode and cathode, resulting in an ionization of the fill gas. The gas ions are accelerated towards the cathode and, upon impact on the cathode, sputter cathode material that is excited in the glow discharge to emit the radiation of the sputtered material, i.e., the element of interest. In the majority of cases single element lamps are used, where the cathode is pressed out of predominantly compounds of the target element. Multi-element lamps are available with combinations of compounds of the target elements pressed in the cathode. Multi element lamps produce slightly less sensitivity than single element lamps and the combinations of elements must be selected carefully to avoid spectral interferences. Most multi-element lamps combine a handful of elements, e.g.: 2 - 8. Atomic Absorption Spectrometers can feature as few as 1-2 hollow cathode lamp positions or in automated multi-element spectrometers, a 8-12 lamp positions may be available.

Electrodeless discharge lamps (EDL) contain a small quantity of the analyte as a metal or a salt in a quartz bulb together with an inert gas, typically argon gas, at low pressure. The bulb is inserted into a coil that is generating an electromagnetic radio frequency field, resulting in a low-pressure inductively coupled discharge in the lamp. The emission from an EDL is higher than that from an HCL, and the line width is generally narrower, but EDLs need a separate power supply and might need a longer time to stabilize.

Deuterium HCL or even hydrogen HCL and deuterium discharge lamps are used in LS AAS for background correction.^[14] The radiation intensity emitted by these lamps decreases significantly with increasing wavelength, so that they can be only used in the wavelength range between 190 and about 320 nm.

When a continuum radiation source is used for AAS, it is necessary to use a high-resolution monochromator, as will be discussed. In addition, it is necessary that the lamp emits radiation of intensity at least an order of magnitude above that of a typical HCL over the entire wavelength range from 190 nm to 900 nm. A special high-pressure xenon short arc lamp, operating in a hot-spot mode has been developed to fulfill these requirements.

As already pointed out, there is a difference between medium-resolution spectrometers that are used for LS AAS and high-resolution spectrometers that are designed for CS AAS. The spectrometer includes the spectral sorting device (monochromator) and the detector.

In LS AAS, the high resolution that is required for the measurement of atomic absorption is provided by the narrow line emission of the radiation source, and the monochromator simply has to resolve the analytical line from other radiation emitted by the lamp.^{[citation needed]} This can usually be accomplished with a band pass between 0.2 and 2 nm, i.e., a medium-resolution monochromator. Another feature to make LS AAS element-specific is modulation of the primary radiation and the use of a selective amplifier that is tuned to the same modulation frequency, as already postulated by Alan Walsh. This way any (unmodulated) radiation emitted for example by the atomizer can be excluded, which is imperative for LS AAS. Simple monochromators of the Littrow or (better) the Czerny-Turner design are typically used for LS AAS. Photomultiplier tubes are the most frequently used detectors in LS AAS, although solid state detectors might be preferred because of their better signal-to-noise ratio.

When a continuum radiation source is used for AAS, a high-resolution monochromator is required. The resolution must be equal to or better than the half-width of an atomic absorption line (about 2 pm) in order to avoid loss of sensitivity and linearity of the calibration graph. Research with high-resolution (HR) CS AAS was pioneered by the groups of O'Haver and Harnly in the US, who also developed the (until recently) only simultaneous multi-element spectrometer for this technique. The breakthrough, however, came when the group of Becker-Ross in Berlin, Germany, built a spectrometer entirely designed for HR-CS AAS. The first commercial equipment for HR-CS AAS was introduced by Analytik Jena (Jena, Germany) at the beginning of the 21st century, based on the design proposed by Becker-Ross and Florek. These spectrometers use a compact double monochromator with a prism pre-monochromator and an echelle grating monochromator for high resolution. A linear charge-coupled device (CCD) array with 200 pixels is used as the detector. The second monochromator does not have an exit slit; hence the spectral environment at both sides of the analytical line becomes visible at high resolution. As typically only 3–5 pixels are used to measure the atomic absorption, the other pixels are available for correction purposes. One of these corrections is for lamp flicker noise, which is independent of wavelength, resulting in measurements with very low noise level; other corrections are for background absorption, to be discussed further.

The relatively small number of atomic absorption lines (compared to atomic emission lines) and their narrow width (a few pm) make spectral overlap rare; there are only few examples known that an absorption line from one element will overlap with another.^{[citation needed]} Molecular absorption, in contrast, is much broader, so that it is more likely that some molecular absorption band will overlap with an atomic line. This kind of absorption might be caused by un-dissociated molecules of concomitant elements of the sample or by flame gases. We have to distinguish between the spectra of di-atomic molecules, which exhibit a pronounced fine structure, and those of larger (usually tri-atomic) molecules that don't show such fine structure. Another source of background absorption, particularly in ET AAS, is scattering of the primary radiation at particles that are generated in the atomization stage, when the matrix could not be removed sufficiently in the pyrolysis stage.

All these phenomena, molecular absorption and radiation scattering, can result in artificially high absorption and an improperly high (erroneous) calculation for the concentration or mass of the analyte in the sample. There are several techniques available to correct for background absorption, and they are significantly different for LS AAS and HR-CS AAS.

In LS AAS background absorption can only be corrected using instrumental techniques, and all of them are based on two sequential measurements:^[15] firstly, total absorption (atomic plus background), secondly, background absorption only. The difference of the two measurements gives the net atomic absorption. Because of this, and because of the use of additional devices in the spectrometer, the signal-to-noise ratio of background-corrected signals is always significantly inferior compared to uncorrected signals. It should also be pointed out that in LS AAS there is no way to correct for (the rare case of) a direct overlap of two atomic lines. In essence, there are three techniques used for background correction in LS AAS:

This is the oldest and still most commonly used technique, particularly for flame AAS. In this case, a separate source (a deuterium lamp) with broad emission is used to measure the background absorption over the entire width of the exit slit of the spectrometer. The use of a separate lamp makes this technique the least accurate one, as it cannot correct for any structured background. It also cannot be used at wavelengths above about 320 nm, as the emission intensity of the deuterium lamp becomes very weak. The use of deuterium HCL is preferable compared to an arc lamp due to the better fit of the image of the former lamp with that of the analyte HCL.

This technique (named after their inventors) is based on the line-broadening and self-reversal of emission lines from HCL when high current is applied. Total absorption is measured with normal lamp current, i.e., with a narrow emission line, and background absorption after application of a high-current pulse with the profile of the self-reversed line, which has little emission at the original wavelength, but strong emission on both sides of the analytical line. The advantage of this technique is that only one radiation source is used; among the disadvantages are that the high-current pulses reduce lamp lifetime, and that the technique can only be used for relatively volatile elements, as only those exhibit sufficient self-reversal to avoid dramatic loss of sensitivity. Another problem is that background is not measured at the same wavelength as total absorption, making the technique unsuitable for correcting structured background.

An alternating magnetic field is applied at the atomizer (graphite furnace) to split the absorption line into three components, the π component, which remains at the same position as the original absorption line, and two σ components, which are moved to higher and lower wavelengths, respectively.^[16] Total absorption is measured without magnetic field and background absorption with the magnetic field on. The π component has to be removed in this case, e.g. using a polarizer, and the σ components do not overlap with the emission profile of the lamp, so that only the background absorption is measured. The advantages of this technique are that total and background absorption are measured with the same emission profile of the same lamp, so that any kind of background, including background with fine structure can be corrected accurately, unless the molecule responsible for the background is also affected by the magnetic field and using a chopper as a polariser reduces the signal to noise ratio. While the disadvantages are the increased complexity of the spectrometer and power supply needed for running the powerful magnet needed to split the absorption line.

In HR-CS AAS background correction is carried out mathematically in the software using information from detector pixels that are not used for measuring atomic absorption; hence, in contrast to LS AAS, no additional components are required for background correction.

It has already been mentioned that in HR-CS AAS lamp flicker noise is eliminated using correction pixels. In fact, any increase or decrease in radiation intensity that is observed to the same extent at all pixels chosen for correction is eliminated by the correction algorithm.^{[citation needed]} This obviously also includes a reduction of the measured intensity due to radiation scattering or molecular absorption, which is corrected in the same way. As measurement of total and background absorption, and correction for the latter, are strictly simultaneous (in contrast to LS AAS), even the fastest changes of background absorption, as they may be observed in ET AAS, do not cause any problem. In addition, as the same algorithm is used for background correction and elimination of lamp noise, the background corrected signals show a much better signal-to-noise ratio compared to the uncorrected signals, which is also in contrast to LS AAS.

The above technique can obviously not correct for a background with fine structure, as in this case the absorbance will be different at each of the correction pixels. In this case, HR-CS AAS is offering the possibility to measure correction spectra of the molecule(s) that is (are) responsible for the background and store them in the computer. These spectra are then multiplied with a factor to match the intensity of the sample spectrum and subtracted pixel by pixel and spectrum by spectrum from the sample spectrum using a least-squares algorithm. This might sound complex, but first of all the number of di-atomic molecules that can exist at the temperatures of the atomizers used in AAS is relatively small, and second, the correction is performed by the computer within a few seconds. The same algorithm can actually also be used to correct for direct line overlap of two atomic absorption lines, making HR-CS AAS the only AAS technique that can correct for this kind of spectral interference.

• Absorption spectroscopy
• Beer–Lambert law
• Inductively coupled plasma mass spectrometry
• Laser absorption spectrometry