Stephen and Timothy Quay (/kweɪ/ KWAY; born June 17, 1947) are American identical twin brothers and stop-motion animators who are better known as the Brothers Quay or Quay Brothers. They received the 1998 Drama Desk Award for Outstanding Set Design for their work on the play The Chairs.

The Quay Brothers reside and work in England, having moved there in 1969 to study at the Royal College of Art, London after studying illustration (Timothy) and film (Stephen) at the Philadelphia College of Art, later the University of the Arts in Philadelphia. In England, they made their first short films, which no longer exist after the only prints were irreparably damaged. They spent some time in the Netherlands in the 1970s and then returned to England, where they teamed up with another Royal College student, Keith Griffiths, who produced all of their films. In 1980, the trio formed Koninck Studios, which is currently based in London's Old Street area of Hackney.

The Brothers' works from 1979 to the present show a wide range of often esoteric influences, starting with the Polish animators Walerian Borowczyk and Jan Lenica and continuing with the writers Franz Kafka, Bruno Schulz, Robert Walser and Michel de Ghelderode, puppeteers Wladyslaw Starewicz and Czech Richard Teschner and Czech composers Leoš Janáček, Zdeněk Liška and Polish Leszek Jankowski, the last of whom has created many original scores for their work. Czech animator Jan Švankmajer, for whom they named one of their films (The Cabinet of Jan Svankmajer), is also frequently cited as a major influence, but they actually discovered his work relatively late, in 1983, by which time their characteristic style and preoccupations had been fully formed. In a panel discussion with Daniel Bird and Andrzej Klimowski at the Aurora festival in Norwich, they emphasized that a more significant influence on their work was Walerian Borowczyk, who made both animation shorts and live-action features.

Most of their animation films feature puppets made of doll parts and other organic and inorganic materials, often partially disassembled, in a dark, moody atmosphere. Perhaps their best known work is Street of Crocodiles (1986), based on the short story of the same name by the Polish author and artist Bruno Schulz. This short film was selected by director and animator Terry Gilliam as one of the ten best animated films of all time, and critic Jonathan Romney included it on his list of the ten best films in any medium (for Sight and Sound's critics' poll of 2002). They have made two full-length live action films: Institute Benjamenta, or This Dream People Call Human Life (1996), produced by Keith Griffiths and Janine Marmot, and The Piano Tuner of Earthquakes (2005), produced by Keith Griffiths. They also directed an animated sequence in the film Frida (2002).

With very few exceptions, their films have no meaningful spoken dialogue (most have no spoken content at all, while some, such as The Comb (From the Museums of Sleep) (1990) include multilingual background gibberish that is not intended to be coherently understood). Accordingly, their films are highly reliant on their music scores, of which many have been written especially for them by the Polish composer Leszek Jankowski. In 2000, they contributed a short film to the BBC's Sound On Film series in which they visualised a 20-minute piece by the avant-garde composer Karlheinz Stockhausen. Whenever possible, the Quay Brothers prefer to work with pre-recorded music, though Gary Tarn's score for The Phantom Museum had to be added afterwards when it became impossible to license music by the Czech composer Zdeněk Liška.

They have created music videos for His Name Is Alive ("Are We Still Married", "Can't Go Wrong Without You"), Michael Penn ("Long Way Down (Look What the Cat Drug In)") and 16 Horsepower ("Black Soul Choir"). Their style has been mimicked to the point that audiences mistakenly believed that the Quay Brothers were responsible for several music videos for Tool but those videos were created by Fred Stuhr and member Adam Jones, whose work is influenced by the Quay Brothers. Although they worked on Peter Gabriel's seminal video "Sledgehammer" (1986) as animators, the project was directed by Stephen R. Johnson in order for the brothers to continue to support their personal projects.

Before turning to film, the Quay Brothers worked as professional illustrators. The first edition of Anthony Burgess' novel The Clockwork Testament, or Enderby's End, included their drawings before the start of each chapter. Nearly three decades before directly collaborating with Stockhausen, they designed the cover of the book Stockhausen: Conversations with the Composer (ed. Jonathan Cott, Simon & Schuster, 1973). After designing book covers for Gothic and science fiction books while in Philadelphia, the Quay Brothers have created suggestive designs for a variety of publications that seem to reflect not only their own interests in particular authors, covers for Italo Calvino, Louis-Ferdinand Céline or Mark le Fanu's study of the films of Andrei Tarkovsky, but also in themes and motifs that these authors develop. Literary texts are inspirational sources for almost all of their film projects, whether they serve as a point of departure for their own ideas or as a textual basis for filmic scenarios, and not as scripts or screenplays. The prowess in illustration and calligraphy seeps increasingly into many formal elements in their later films, evident as graphic embellishment in the set decoration, or their particular use of patterns in the puppets' costume design. Titles, intertitles and credits appear in a variety of handwritten styles.

In an interview with Robert K. Elder for his book The Best Film You've Never Seen, the Quay Brothers discuss their creative process, stating: "If [a] project does eventually get approval, then we almost invariably chuck [the] original proposal out, not out of any cavalierness, but simply because we know that, as we start building the decors and the puppets, the script begins to grow and evolve very organically."