The ionization chamber is the simplest type of gaseous ionisation detector, and is widely used for the detection and measurement of many types of ionizing radiation, including X-rays, gamma rays, alpha particles and beta particles. Conventionally, the term "ionization chamber" refers exclusively to those detectors which collect all the charges created by direct ionization within the gas through the application of an electric field. It uses the discrete charges created by each interaction between the incident radiation and the gas to produce an output in the form of a small direct current. This means individual ionising events cannot be measured, so the energy of different types of radiation cannot be differentiated, but it gives a very good measurement of overall ionising effect.

It has a good uniform response to radiation over a wide range of energies and is the preferred means of measuring high levels of gamma radiation, such as in a radiation hot cell as they can tolerate prolonged periods in high radiation fields without degradation. They are widely used in the nuclear power industry, research labs, fire detection, radiation protection, and environmental monitoring.

A gas ionization chamber measures the charge from ion pairs created within a gas caused by incident radiation. It consists of a gas-filled chamber with two electrodes; known as anode and cathode. The electrodes may be in the form of parallel plates (Parallel Plate Ionization Chambers: PPIC), or a cylinder with a coaxially located internal anode wire.

A voltage potential is applied between the electrodes to create an electric field in the fill gas. When gas atoms or molecules between the electrodes are ionized by incident ionizing radiation, ion-pairs are created and the resultant positive ions and dissociated electrons move to the electrodes of the opposite polarity under the influence of the electric field. This generates an ionization current which is measured by an electrometer circuit in the region of femtoamperes to picoamperes, depending on the chamber design, and is proportional to the radiation dose.

The electric field has to be sufficiently strong to prevent the recombination of ion pairs which would diminish the ion current, and build-up of positive ions is prevented by their recombination with electrons when they reach the cathode. This mode of operation is referred to as "current" mode, meaning that the output signal is a continuous current, and not a pulse output as in the cases of the Geiger–Müller tube or the proportional counter.

Referring to the accompanying ion-pair collection graph, it can be seen that in the ion chamber operating region the charge of a collected ion pair is effectively constant over a range of applied voltage, as due to its relatively low electric field strength the ion chamber does not have any multiplication effect. This is in distinction to the Geiger–Müller tube or the proportional counter whereby secondary electrons, and ultimately multiple avalanches, greatly amplify the original ionisation charges to produce measurable pulses.

The following chamber types are commonly used.

This is a chamber freely open to atmosphere, where the fill gas is ambient air. The domestic smoke detector is a good example of this, where a natural flow of air through the chamber is necessary so that smoke particles can be detected by the change in ion current. Other examples are applications where the ions are created outside the chamber but are carried in by a forced flow of air or gas.