Levi Strauss never married and had no direct descendants. He remained a bachelor throughout his life, dedicating his time and energy to his business and philanthropic endeavors.

Levi maintained close relationships with his siblings and their families. He considered his nephews and nieces to be his family, taking an active interest in their lives and providing them with opportunities.

Levi Strauss served as a mentor to his nephews, particularly Jacob, Sigmund, Louis, and Abraham Stern, who joined Levi Strauss & Co. and played key roles in the company's management. He entrusted them with the responsibility of carrying on his legacy.