The Negative Zone is a fictional setting, an antimatter universe appearing in American comic books published by Marvel Comics. The location is depicted in various publications from Marvel, most frequently in Fantastic Four and Captain Marvel. Created by Stan Lee and Jack Kirby, it first appeared in Fantastic Four #51 (June 1966).

The Negative Zone in the Marvel Universe is a universe parallel to Earth's. While the universes are similar in many respects they are different in that: all matter in the Negative Zone is negatively charged; the Negative Zone is entirely filled with a pressurized, breathable atmosphere; and near the center of the Negative Zone is a deadly vortex of unspeakable power. Since the Negative Zone is largely uninhabited, several would-be conquerors have attempted to bridge the gap to Earth and take over its population. Notable residents of the Negative Zone include Blastaar and Annihilus.

The Negative Zone is often visited by the Fantastic Four as Mister Fantastic discovered it, and has mapped portions of it extensively. The Age of Apocalypse version of Blink also visited it once, proving that there is only one Negative Zone that does not change with the multiverse.

For a number of years, Mar-Vell and Rick Jones were bonded to each other, causing one of them to exist in the Negative Zone while the other would exist in the regular universe. They exchanged places by clasping the special bracelets each wore or automatically after a few hours.

Spider-Man has also visited the Negative Zone, and acquired a costume that allowed him to merge with shadows and become practically invisible.^{[volume and issue needed]} When he was framed by Norman Osborn a few weeks later, he used the costume to become Dusk (one of his four new superhero identities during the Identity Crisis story arc).^{[volume and issue needed]} A few months later after Spider-Man's name was cleared, Cassie St. Commons assumed the identity of Dusk and joined the Slingers.^{[volume and issue needed]} Cletus Kasady also visited the Negative Zone, finding and bonding with a symbiote there, as he had lost the original Carnage symbiote when Venom absorbed it into his own symbiote.^{[volume and issue needed]}

The Negative Zone 1.5 million years ago, nearly coinciding with the rise of the Skrull and Kree races. It is believed that around that time the Negative Zone ceased expanding and began its "Big Crunch", contracting toward a central nexus. Some of the most powerful and influential races thereby faced destruction and sought to preserve their lives, including the Tyannans. During this time, Blastaar and Annihilus rise to power.

While searching for a way to travel through sub-space, Reed Richards discovers a gateway to the Negative Zone. After studying the Negative Zone using drones, Reed concludes that it is largely unpopulated. He—and others—use the Negative Zone on several occasions to get rid of difficult enemies, such as the Super-Adaptoid and Galactus. In Civil War, Iron Man, Mister Fantastic and Yellowjacket create a massive prison in the Negative Zone to house captured non-registered heroes as they await trial. It is designated Negative Zone Prison Alpha and also known as "Project 42", as it had been the 42nd idea out of a hundred that the three created.

In Secret Invasion: Fantastic Four, the Skrull warrior Lyja (posing as Susan Richards) sends the Baxter Building into the Negative Zone. She reveals herself to Johnny Storm (her former spouse) and attacks him, feeling angry that he had forgotten her. Johnny saves Lyja from being hit by a police car, but they are both pulled in through a portal. The two reconcile and fend off a Negative Zone creature that attacks them. Lyja refuses to leave the Negative Zone, intending to find out who she is.