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Nitrogen fixation

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Nitrogen fixation

Nitrogen fixation is a chemical process by which molecular dinitrogen (N
2
) is converted into ammonia (NH
3
). It occurs both biologically and abiologically in chemical industries. Biological nitrogen fixation or diazotrophy is catalyzed by enzymes called nitrogenases. These enzyme complexes are encoded by the Nif genes (or Nif homologs) and contain iron, often with a second metal (usually molybdenum, but sometimes vanadium).

Some nitrogen-fixing bacteria have symbiotic relationships with plants, especially legumes, mosses and aquatic ferns such as Azolla. Looser non-symbiotic relationships between diazotrophs and plants are often referred to as associative, as seen in nitrogen fixation on rice roots. Nitrogen fixation occurs between some termites and fungi. It occurs naturally in the air by means of NOx production by lightning.

Fixed nitrogen is essential to life on Earth. Organic compounds such as DNA and proteins contain nitrogen. Industrial nitrogen fixation underpins the manufacture of all nitrogenous industrial products, which include fertilizers, pharmaceuticals, textiles, dyes and explosives.

Biological nitrogen fixation was discovered by Jean-Baptiste Boussingault in 1838. Later, in 1880, the process by which it happens was discovered by German agronomist Hermann Hellriegel and Hermann Wilfarth [de] and was fully described by Dutch microbiologist Martinus Beijerinck.

"The protracted investigations of the relation of plants to the acquisition of nitrogen begun by de Saussure, Ville, Lawes, Gilbert and others, and culminated in the discovery of symbiotic fixation by Hellriegel and Wilfarth in 1887."

"Experiments by Bossingault in 1855 and Pugh, Gilbert & Lawes in 1887 had shown that nitrogen did not enter the plant directly. The discovery of the role of nitrogen fixing bacteria by Herman Hellriegel and Herman Wilfarth in 1886–1888 would open a new era of soil science."

In 1901, Beijerinck showed that Azotobacter chroococcum was able to fix atmospheric nitrogen. This was the first species of the azotobacter genus, so-named by him. It is also the first known diazotroph, species that use diatomic nitrogen as a step in the complete nitrogen cycle.

Biological nitrogen fixation (BNF) occurs when atmospheric nitrogen is converted to ammonia by a nitrogenase enzyme. The overall reaction for BNF is:

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