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Prokaryote

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Prokaryote

A prokaryote (/prˈkærit, -ət/; less commonly spelled procaryote) is a microorganism whose usually single cell lacks a nucleus or other membrane-bound organelles. The word prokaryote comes from the Ancient Greek πρό (pró), meaning 'before', and κάρυον (káruon), meaning 'nut' or 'kernel'. In the earlier two-empire system, prokaryotes formed the empire Prokaryota. In the three-domain system, based upon molecular phylogenetics, prokaryotes are divided into two domains: Bacteria and Archaea. A third domain, Eukaryota, consists of organisms with nuclei.

Prokaryotes evolved before eukaryotes, and lack nuclei, mitochondria, and most of the other distinct organelles that characterize the eukaryotic cell. Some unicellular prokaryotes, such as cyanobacteria, form colonies held together by biofilms, and large colonies can create multilayered microbial mats. Prokaryotes are asexual, reproducing via binary fission. Horizontal gene transfer is common as well.

Molecular phylogenetics has provided insight into the interrelationships of the three domains of life. The division between prokaryotes and eukaryotes reflects two very different levels of cellular organization; only eukaryotic cells have an enclosed nucleus that contains their DNA, and other membrane-bound organelles including mitochondria. More recently, the primary division has been seen as that between Archaea and Bacteria, since eukaryotes may be part of the archaean clade and have multiple homologies with other Archaea.

The cellular components of prokaryotes are not enclosed in membranes within the cytoplasm, like eukaryotic organelles. Bacteria have microcompartments, quasi-organelles enclosed in protein shells such as encapsulin protein cages, while both bacteria and some archaea have gas vesicles.

Prokaryotes have simple cell skeletons. These are highly diverse, and contain homologues of the eukaryote proteins actin and tubulin. The cytoskeleton provides the capability for movement within the cell.

Most prokaryotes are between 1 and 10 μm, but they vary in size from 0.2 μm in Thermodiscus spp. and Mycoplasma genitalium to 750 μm in Thiomargarita namibiensis.

Bacterial cells have various shapes, including spherical or ovoid cocci, e.g., Streptococcus; cylindrical bacilli, e.g., Lactobacillus; spiral bacteria, e.g., Helicobacter; or comma-shaped, e.g., Vibrio. Archaea are mainly simple ovoids, but Haloquadratum is flat and square.

Bacteria and archaea reproduce through asexual reproduction, usually by binary fission. Genetic exchange and recombination occur by horizontal gene transfer, not involving replication. DNA transfer between prokaryotic cells occurs in bacteria and archaea.

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