Prostration is the gesture of placing one's body in a reverentially or submissively prone position. Typically prostration is distinguished from the lesser acts of bowing or kneeling by involving a part of the body above the knee, especially the hands, touching the ground.

Major world religions employ prostration as an act of submissiveness or worship to an entity or to the Supreme Being (i.e. God), as in the metanoia in Christian prayer used in the Eastern Orthodox and Oriental Orthodox Churches, and in the sujud of the Islamic prayer, salat. In various cultures and traditions, prostrations are similarly used to show respect to rulers, civil authorities and social elders or superiors, as in the Chinese kowtow or Ancient Greek proskynesis. The act has often traditionally been an important part of religious, civil and traditional rituals and ceremonies, and remains in use in many cultures.

Many religious institutions (listed alphabetically below) use prostrations to embody the lowering, submitting or relinquishing of the individual ego before a greater spiritual power or presence.

In the Baháʼí Faith, prostrations are performed as a part of one of the alternatives of obligatory prayer (the "Long" one) and in the case of traveling, a prostration is performed in place of each missed obligatory prayer in addition to saying "Glorified be God, the Lord of Might and Majesty, of Grace and Bounty". However, if unable to do so, saying "Glorified be God" is sufficient. There are specifics about where the prostration can take place including, "God hath granted you leave to prostrate yourselves on any surface that is clean ..." (note #10) and "He also condemns such practices as prostrating oneself before another person and other forms of behaviour that abase one individual in relation to another". (note #57)

In Buddhism, prostrations are commonly used and the various stages of the physical movement are traditionally counted in threes and related to the Triple Gem, consisting of:

In addition, different schools within Buddhism use prostrations in various ways, such as the Tibetan tantric preliminary practice of a 100,000 prostrations as a means of overcoming pride (see Ngöndro). Tibetan pilgrims often progress by prostrating themselves fully at each step, then moving forward as they get up, in such a way that they have lain on their face on each part of their route. Each three paces involves a full prostration; the number three is taken to refer to the Triple Gem. This is often done round a stupa, and in an extremely arduous pilgrimage, Mount Kailash is circumnavigated entirely by this method, which takes about four weeks to complete the 52-kilometre route. It is also not unusual to see pilgrims prostrating all the way from their home to Lhasa, sometimes a distance of over 2000 km, the process taking up to two years to complete.

In Oriental Orthodox Christianity and Western Orthodox Christianity, believers prostrate during the seven fixed prayer times; prayer rugs are used by some adherents to provide a clean space for believers to offer their Christian prayers to God, e.g. the canonical hours. Oriental Orthodox Christians, such as Copts, incorporate prostrations in their prayers that are performed facing eastward in anticipation of the Second Coming of Jesus, "prostrating three times in the name of the Trinity; at the end of each Psalm … while saying the ‘Alleluia’; and multiple times" during the forty-one Kyrie eleisons" (cf. Agpeya). Syriac Orthodox and Indian Orthodox Christians, as well as Christians belonging to the Mar Thoma Syrian Church (an Oriental Protestant denomination), make multiple prostrations at the seven fixed prayer times during which the canonical hours are prayed, thrice during the Qauma prayer, at the words "Crucified for us, Have mercy on us!", thrice during the recitation of the Nicene Creed at the words "And was incarnate of the Holy Spirit...", "And was crucified for us...", & "And on the third day rose again...", as well as thrice during the Prayer of the Cherubim while praying the words "Blessed is the glory of the Lord, from His place forever!" (cf. Shehimo). Oriental Catholic and Oriental Protestant rites also use prostrations in a similar way as the Oriental Orthodox Churches.

Among Old Ritualists, a prayer rug known as the Podruchnik is used to keep one's face and hands clean during prostrations, as these parts of the body are used to make the sign of the cross.