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Othram (also Othram Inc.) is an American corporation specializing in forensic genetic genealogy to resolve unsolved murders, disappearances, and identification of unidentified decedents or murder victims (colloquially known as John Does and Jane Does).[1] The company offers law enforcement agencies tools and programs to infer kinship among individuals, closely and distantly related, through a combination of short tandem repeat and single nucleotide polymorphism testing,[2] as well as forensic genome sequencing of DNA.[2]

Key Information

Othram has assisted with identifications of cold cases such as Beth Doe, Septic Tank Sam, and Delta Dawn.[3][4] Many cases are not publicized until after a successful identification.

Othram technology and casework inspired the 500th episode of Law & Order: Special Victims Unit.[5][6]

Notable cases

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Suspect identifications

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These are cases where Othram has sequenced DNA and returned forensic genetic genealogy leads for the suspect in a violent crime, such as a sexual assault or homicide.

Murder of Carla Walker

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Carla Walker was a 17-year-old girl who was abducted from a bowling alley in Fort Worth, Texas, on February 15, 1974. She and her boyfriend, Rodney McCoy, had been sitting in her car outside of the alley after attending a school dance at Western Hills High School when they were attacked by an unknown assailant who pistol whipped Roy, leaving him unconscious.[7] Walker's body was later recovered three days later from a cow culvert, half an hour south of Fort Worth. She had been beaten, tortured, raped and strangled to death, as well as injected with morphine.[8]

Othram Inc. was contacted in April or May 2020 to use forensic genealogy on DNA left on Walker's body to identify her killer. On September 21, 2020, it was announced that Glen Samuel McCurley, 77 at the time of his arrest, was charged with Walker's murder based on a DNA match.[9]

Murder of Christine Jessop

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Christine Jessop was a 9-year-old girl from Queensville, Ontario, who was abducted after getting off her school bus in October 1984. She went home, dropped off her bag, and planned on meeting with a friend at a nearby park. She never made it to the park. Her body was later recovered on December 31 in a farmer's field in Sunderland, Ontario. She had been raped and stabbed to death, with semen being found on her underwear.[10]

In 2019, the Toronto Police Service contacted Othram to generate a DNA profile from the semen found on Jessop's clothing and perform forensic genealogy to find her killer. After Othram was able to successfully sequence a profile, Toronto Police were able to find her killer in the fall. It was announced on October 15, that Calvin Hoover, a man who died in 2015, matched the profile of DNA and was named as her killer.[11]

Murder of Siobhan McGuinness

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Siobhan McGuinness was a 5-year-old girl from Missoula, Montana, who was abducted in early 1974 while walking to a friend's house. Her body was found two days later in a snowy culvert. She had been raped and stabbed to death. An extensive investigation between law enforcement and the community was made in order to find her killer until all leads were exhausted.

In 2020, the Missoula Police Department, BODE Technology, and the FBI contacted Othram to create a genetic profile from DNA found on the crime scene, after similar techniques were used to identify the Golden State Killer. After a profile was made, the team used forensic genealogy to find Richard William Davis, who was confirmed by DNA to be her killer. Davis had died in 2012.[12]

Unidentified remains cases

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These are cases where Othram has sequenced DNA and used forensic genetic genealogy to identify an unidentified decedent.

Alisha Heinrich AKA "Delta Dawn"

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On December 5, 1982, the body of a female toddler was seen floating in the Escatawpa River beneath a bridge on Interstate 10 in Moss Point, Mississippi. She had reportedly been seen a few days earlier with her mother, then-unidentified Gwendolyn Clemmons, as Clemmons paced the bridge, seemingly distressed. Drivers reported seeing Clemmons' body floating in the river, but when law enforcement conducted a search, they instead found Heinrich, deceased. During subsequent searches, another man was recovered; however, he was found to be unrelated to the case. Clemmons has not been found, and is believed to be deceased.

Othram was contacted by the Jackson County Sheriff's Office in 2019 to create a genetic profile for genetic genealogy. After the profile was developed, the Sheriff's Office and FBI worked together and were able to identify Heinrich in September 2020. On December 4, 2020, it was announced that "Delta Dawn" had been identified as Alisha Ann Heinrich, last seen with her mother in Kansas City, Missouri.[13]

Vance Rodriguez AKA "Mostly Harmless"

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Reconstruction of Vance Rodriguez

On July 23, 2018, a hiker was found dead in his tent near the Appalachian Trail in Big Cypress National Preserve in Florida; he appeared to have starved to death. He was estimated to be between 35 and 60 years old, was 5 feet 8 inches (1.73 m) tall and weighed 83 pounds (38 kg). He had graying dark brown hair, an unkempt gray and brown beard, and blue eyes. He also appeared to have a faint scar across his abdomen. Rodriguez was found wearing a beige shirt with neon green and gray accents, gray "Russel" brand shorts, "Performance" brand underwear, and a Columbia baseball cap. He was also found with various camping and hiking items. He had been seen alive various times previously, and went by the names "Mostly Harmless", "Ben Bilemy", and "Denim" to those he met, possibly referencing Douglas Adams' Hitchhiker's Guide to the Galaxy.[14]

Othram Inc. was contacted in July 2020 to help identify "Mostly Harmless". On January 12, 2021, it was announced that "Mostly Harmless" had been identified as Vance John Rodriguez of Lafayette Parish, Louisiana, who had moved to Brooklyn, New York, after he was recognized by a former co-worker after seeing a flyer.

Evelyn Colon AKA "Beth Doe"

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Original sketch of Evelyn Colon

On December 20, 1976, the dismembered body of a young white or Hispanic female was found in multiple suitcases beneath a highway bridge in White Haven, Pennsylvania. Investigators were unable to identify her and she was buried under the name "Beth Doe".[15] After 44 years, investigators finally got a break in the case in 2020 when they compared her DNA to genealogy databases and identified Luis Colon Jr. as a relative of the deceased. Colon Jr. and his family told investigators that Colon Jr.'s aunt, Evelyn Colon, disappeared from Jersey City, New Jersey, at the age of 15 in December 1976 and that she was pregnant and in an abusive relationship at the time she disappeared.

In 2020, the Pennsylvania State Police and the NCMEC contacted Othram to sequence an extraction of DNA in order for them to use forensic genealogy.[3] On March 31, 2021, investigators publicly announced that Beth Doe had been identified as Evelyn Colon.[16] Luis Sierra, Colon's boyfriend and the father of her child, was arrested for her murder.[17]

Janet Lucas AKA "Christy Crystal Creek"

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External image
image icon Photo of Janet Lucas with 3D Reconstruction

On September 9, 1985, the skeleton of a young woman, nicknamed "Christy Crystal Creek," was found in Missoula, Montana with two .32 caliber bullets in her skull. No clothing was located at the crime scene. Investigators believed this individual may have died sometime between 1983 and early 1985.[18] She stood between 4 feet 10 inches and 5 feet 2 inches (1.47–1.57 m), weighing between 90 and 110 pounds (41–50 kg). Examination indicated that she had a history of smoking and had many fillings as well as two root canals. She also had a type of oral surgery which is distinct to Asia's dental techniques, involving the screwing of a dental post into the tooth. Along with other physical characteristics, it was previously thought she was possibly of Asian descent.[19] It is possible that serial killer and rapist Wayne Nance was responsible for her murder; due to his death in 1986, he was never tried or convicted of any murder.[19][20][21] Two other victims believed to have been killed by Nance are "Debbie Deer Creek" and "Betty Beavertail," later identified as Marcella Bachmann and Devonna Nelson, respectively. He also may have committed the murders of two others prior to the Jane Doe discoveries.[22]

Lucas was identified in May 2021 with assistance from Othram. She was last seen in Idaho in 1983 and was originally from Spokane, Washington.[23]

Gordon "Gordie" Sanderson AKA "Septic Tank Sam"

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On April 13, 1977, the decomposing remains of a young to middle aged Native American or white man were found by residents searching a 1.8 meter-deep septic tank in Tofield, Alberta. He had been shot several times and had been sexually mutilated and tortured beforehand, including being burned with a blowtorch and cigarette lighters. Investigators think that the killer must have been familiar with the area, due to the property being derelict and in a very rural location. He was estimated to be between 26 and 50 years old, was 168 cm tall and weighed 70 kilograms. He had brown hair and notable recent dental work. He was found wearing a Levi work shirt with snap buttons, a gray T-shirt, a pair of jeans, a pair of gray wool socks and a pair of brown imitation 'Wallabee' shoes.[24]

Othram was contacted by the Alberta RCMP and Office of the Chief Medical Examiner in 2020 to develop new leads on his identity or killer. With the new genetic profile, "Septic Tank Sam" was identified in June 2021 as Gordon "Gordie" Edwin Sanderson of Edmonton, Alberta.[4]

Margaret Fetterolf AKA "Woodlawn Jane Doe"

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On September 12, 1976, the body of a young woman was found partially wrapped in a white sheet near a cemetery in Woodlawn, Baltimore County, Maryland. She had been beaten, strangled, and raped. The rape had caused bleeding that had seeped into her clothing. Investigators speculated that she was murdered at a different location and then left at the scene at which she was found. It was estimated that she was between 15 and 30 years old, 149 and 159 pounds, and 5 feet 6 to 5 feet 9 inches tall. She was found wearing a white and tan shirt, a white bra, and knee-high socks with multi-colored stripes.

Othram was contacted by BODE Technology and Baltimore County Police in February 2021 to produce a comprehensive genealogical profile from DNA extracted from the victim. On September 15, 2021, it was announced that she had been identified as 16-year-old Margaret Fetterolf of Alexandria, Virginia.[25]

Clara Birdlong AKA "Escatawpa Jane Doe"

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On December 27, 1977, skeletal human remains were found in Escatawpa, Mississippi. The victim was determined to be an African American woman with a front gold tooth. She possibly wore a wig. It is estimated that the woman might have died 3 to 4 months before she was found.

In 2018, serial killer Samuel Little confessed to numerous murders, including the murder of Escatawpa Jane Doe. Little said he murdered a woman that he met at a bar in Gulfport and that she was possibly from the Pascagoula area. Investigators confirmed that he was arrested in Pascagoula in August 1977 during the time period the victim was estimated to have died. He died in prison in December 2020.

The Jackson County Sheriff's Office, in cooperation with the Mississippi State Crime Lab, reached out to Othram to use advanced DNA testing to identify the victim. On September 21, 2021, it was announced that she had been identified as 44-year-old Clara Birdlong from Leflore County, Mississippi.[26]

George Seitz AKA "Queens John Doe"

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On March 12, 2019, the dismembered partial remains of a man were discovered in the backyard of a home in Queens, New York City, after a woman contacted police and informed them that she had seen her stepfather burying a body when she was a child in the 1970s. However, she did not know the identity of the victim.

Othram partnered with the Queens District Attorney's Office and the NYC FBI to identify the victim. On November 3, 2021, it was announced that he had been identified as 81-year-old George Clarence Seitz, who disappeared after leaving his home to get a haircut on December 10, 1976. Seitz was a World War I veteran. Martin Motta was arrested and indicted by a grand jury for the murder.[27]

Sherri Jarvis AKA "Walker County Jane Doe"

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On November 1, 1980, the body of a teenage girl was found along Interstate 45 near Huntsville, Texas, having been beaten severely and strangled. 14-year-old Sherri Ann Jarvis had been in the custody of the state of Minnesota, after excessive truancy from school, until she ran away and disappeared at age 14. It is not known how she made her way to Texas, but three witnesses in the area described her as saying she wanted to go to the now-defunct Ellis Prison Farm to see a "friend". The connection Sherri may have had to the Ellis Prison Farm has never been discovered.

She was publicly identified on November 9, 2021, with assistance from Othram, Inc. The company had begun work on the case during the summer of 2020, finding a potential match in March 2021. Further investigation led to the victim's positive identification.[28][29]

Sharon Lee Gallegos AKA "Little Miss Nobody"

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The partially buried body of a female child was found in Sand Wash Creek Bed on Old Alamo Road in Congress, Arizona on July 31, 1960. Her cause of death was never determined by medical examiners, but her case was officially declared to be a homicide. She became known as "Little Miss Nobody" after no family or friends came forward to either report her missing or to claim her body. In March 2022, local authorities, with the help of Othram, announced her identity.[30] Gallegos was last seen on 21 July 1960, when she was abducted as she was in an alley behind her home.

Joyce Marilyn Meyer Sommers AKA "Christmas Tree Lady"

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In December 1996, a groundskeeper at Pleasant Valley Memorial Park Cemetery in Annandale, Virginia, found a woman's body. She was found in a section designated for infant burials, but wasn't near a particular grave. The woman was estimated to be a white female with red or copper hair between the ages of 50–70 years old and was approximately 5 feet tall. The woman was found with a plastic bag over her head, along with two fifty dollar bills, one for the coroner and one for the cemetery. A note was found with her body that stated: "Deceased by own hand...prefer no autopsy. Please order cremation with funds provided. Thank you. Jane Doe". The woman's autopsy report showed that she had alcohol and valium in her system when she died. Investigators suspected that the woman committed suicide and the official cause of death was determined to be suffocation. She was given the name of "Christmas Tree Lady" because of a small 8 inch Christmas tree that was found next to her.

Over the years, numerous missing person reports from the National Capital Region were compared to the decedent's physical description, but no matches were found. A colorized sketch of the decedent was made in 2000 in hopes that a friend or family member would recognize her, but no leads were produced. In January 2022, detectives of the Fairfax County Police Department sent physical evidence to Othram where its scientists created a genealogical profile for the decedent. In May 2022, investigative leads were returned to the detectives who used them to find a suspected family member which led to more family connections across the country. A DNA sample from a close relative confirmed a match eventually leading to the decedent's siblings.

On July 7, 2022, the "Christmas Tree Lady" was formally identified as 69-year-old Joyce Marilyn Meyer Sommers. Meyer was born in July 1927, the eldest of 5 siblings and was raised on a farm outside of Davenport, Iowa. Family members believe she moved to Virginia sometime after the mid-1980's. Meyer wasn't reported missing at the time of her death, but family members spent years looking for her and even hired a private detective at one point. It was determined that Meyer never had children.[31]

Tracy Walker AKA "Elk Valley Jane Doe/Baby Girl"

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On April 3, 1985, the skeletonised partial remains of a young girl were discovered about 200 yards off Big Wheel Gap Road, four miles southwest of Jellico, Tennessee, in Campbell County near a strip mine. She was believed to have been dead between one and four years. Her age was estimated between 9 and 15. She was found by a passerby.[32]

On August 30, 2022, she was identified as 15-year-old Tracy Sue Walker of Lafayette, Indiana. The connection was made after Othram Laboratories located a possible family member in the Lafayette area and TBI intelligence analysts located several relatives there, who confirmed they had a relative who disappeared in 1978. DNA samples were taken and submitted to CODIS, from with the UNTCHI identified Walker's remains.[33]

Ruth Marie Terry AKA "Lady of the Dunes"

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In 1974, the decomposing body of a woman was found in Massachusetts. The victim's hands were missing, and her head was nearly severed from her body. She became known as "Lady of the Dunes".

In 2022, skeletal remains were sent to Othram. Despite DNA damage from formaldehyde and other chemicals, a comprehensive DNA profile was built using Forensic-Grade Genome Sequencing®, which was returned to FBI investigators.[34] After discovering a close relative, the FBI were able to confirm that Lady of the Dunes was Ruth Marie Terry.[35] In August 2023, authorities announced that her husband, Guy Muldavin, had killed her, and have now closed the case.[36]

Amore Wiggins AKA "Opelika Jane Doe"

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On January 28, 2012, the skull of a young girl was found in a trailer park in Opelika, Alabama, with additional bones and clothing being found in an adjacent lot. Analysis of the remains determined that they belonged to a young black girl who had likely been malnourished and physically abused while still alive.

On January 19, 2023, Othram identified the decedent as 6-year-old Amore Joveah Wiggins, the daughter of Navy officer Lamar Vickerstaff Jr. and Sherry Wiggins. According to Wiggins, Vickerstaff obtained legal custody of their daughter in 2009 and moved in with his current wife, Ruth, and she never heard from her daughter again. Both of the Vickerstaffs were arrested after the identification, with Lamar facing felony murder charges while Ruth is facing failure to report a missing child.[37]

Kerry Cummings AKA "Torso Girl"

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On October 26, 1997, a human torso was found by a duck hunter in a slough near Eureka, California. Additional remains linked to this victim were located the following year as well. In November 1998, serial killer Wayne Adam Ford confessed to murdering this woman and three others, for which he was tried, convicted and sentenced to death. Despite his confession, this victim's identity remained a mystery.

With the help of a DNA profile and forensic genealogy, Othram managed to identify "Torso Girl" as 25-year-old Kerry Ann Cummings, a woman from Eugene, Oregon, who was known to suffer from an unspecified mental illness and often couch surfed.[38]

Ronald Eugene Woodham IV AKA "Baby Boy Horry"

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In 2008, a newborn baby boy was found in Conway, South Carolina. With few leads to work from, neither the infant nor his parents could be identified.

In 2019, forensic evidence was submitted to Othram, Othram produced investigative leads that were returned to law enforcement investigators. Aided by these leads, law enforcement was able to identify both of the parents as well as the child. His mother was arrested and charged with child abuse homicide, and later accepted a plea deal for manslaughter and was sentenced to 4 years in prison.

The infant was posthumously named Ronald Eugene Woodham IV by his father.

Karen Vergata AKA "Fire Island Jane Doe"

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On April 20, 1996, a pair of legs were found in a plastic bag that had washed ashore in Davis Park, New York. On April 11, 2011, a skull was found west of Tobay Beach near a set of dismembered remains - the skull was later matched to the legs found back in 1996, and this victim was nicknamed "Fire Island Jane Doe". Due to the way in which they were murdered, investigators believed the remains belonged to potential victims of a serial killer dubbed the "Long Island Serial Killer", or simply LISK.

On August 4, 2023, Othram identified the decedent as 34-year-old Karen Vergata, a resident of Manhattan and possible escort who went missing on February 14, 1996. A man named Rex Heuermann has been charged with four of the cases linked to the LISK, but so far has not been publicly named a suspect in this case.[39]

Suzanne Kjellenberg AKA "Suzanne Jane Doe"

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On September 15, 1994, the skeletal remains of a female were found in Holt, Florida, with indications that she had likely been murdered. Serial killer Keith Hunter Jesperson confessed to her murder, claiming that she introduced herself as simply "Suzanne" and that he had met her at a truck stop in Tampa.

In October 2023, Othram identified the decedent as 34-year-old Suzanne L. Kjellenberg of Wisconsin. Investigators have indicated that they would file charges against Jesperson in this case.[40]

Kenyatta Odom AKA "Christmas/Waycross Jane Doe"

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Four days before 1988's Christmas, a truck driver found a human child's remains in a TV cabinet while searching for furniture. She was determined to be African American and speculated to around 3 or 4 years old. In 2020, DNA was submitted to Othram. DNA and isotope forensics revealed that she was likely from Albany, Georgia.

About three years later, someone recognized her facial reconstruction and submitted a tip. Once GBI interviewed and tested her potential family, they can conclusively unearthed her name as Kenyatta Odom. Her mother and her boyfriend at the time of the girl's death were arrested.[41][42][43]

Ruth Waymire AKA "Millie Doe"

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On June 20, 1984, two fishermen fishing near the Spokane River found the dismembered remains of a woman. Her hands and feet had been removed, and she had been decapitated. Her skull was found more than a decade later in April 1998, but this failed to bring new clues to her identity.

The case was revisited by investigators over the years, and Othram eventually managed to find a familial match with sisters living in the Midwest. Through them, the decedent was identified as 24-year-old Ruth Belle Waymire, a Spokane resident who lost contact with her family in 1984. Her second husband, Trampas D.L. Vaughn, who had served prison time in Iowa, is currently the only publicly identified suspect. He cannot be questioned, as he died in Sacramento, California in 2017. Investigators are searching for any information on Waymire, her husband and children.[44]

See also

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References

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Revisions and contributorsEdit on WikipediaRead on Wikipedia
from Grokipedia
Othram Inc. is an American biotechnology company founded in 2018 by David and Kristen Mittelman and headquartered in , specializing in and advanced to resolve cold cases, identify human remains, and locate missing persons. The company operates a purpose-built that vertically integrates DNA extraction, enrichment, sequencing, and genealogical analysis, enabling the processing of challenging evidence samples such as degraded or trace DNA from crime scenes. Othram's proprietary technologies, including for kinship analysis and DNASolves® for building investigative genetic databases, allow agencies to generate leads in previously unsolvable cases by comparing forensic profiles to public and private databases. Unlike traditional DNA testing methods limited to short tandem repeats (STRs), Othram employs sequencing (MPS) to produce whole-genome data, facilitating mixture and identification even from mixed or low-quantity samples. The company's mission focuses on advancing justice through in-house, accredited workflows that maintain from evidence receipt to courtroom-ready results, partnering with organizations like the National Missing and Unidentified Persons System () and international law enforcement. Since its inception, Othram has contributed to solving thousands of cases as of 2025, including high-profile investigations such as the and numerous unidentified remains identifications across the and beyond. Funding through grants, , and donations supports work on underserved cases via initiatives like DNASolves.

Company overview

Founding and leadership

Othram was founded in 2018 by David Mittelman, a computational biologist and with extensive experience in . Mittelman earned his PhD in from and previously contributed to the while at the University of Texas Southwestern Medical Center, later serving as faculty at where he focused on applying computational methods to genomic data. His background in developing tools for analyzing complex biological datasets informed the company's inception. Mittelman's motivation for establishing Othram stemmed from recognized limitations in traditional , particularly the challenges posed by degraded or low-quantity samples common in cold cases, which often yielded insufficient profiles for identification. After two decades addressing medical challenges, he pivoted to forensics to bridge these gaps and enable resolutions for long-stalled investigations. Key leadership includes co-founder Kristen Mittelman, who serves as Chief Development Officer and oversees operational aspects, bringing her expertise in chemistry from a . David Mittelman remains the CEO, guiding strategic direction. The initial vision centered on creating a vertically integrated dedicated to forensic sequencing and , allowing Othram to handle the full pipeline from evidence processing to analysis in-house and support through proprietary tools like the DNASolves database, reducing dependence on external public resources.

Headquarters and operations

Othram is headquartered at 2829 Technology Forest Boulevard, Suite 100, in , a suburb north of , where it established its primary facilities in 2018. The company's operations center around specialized, accredited laboratories designed for , featuring high-throughput sequencing capabilities with equipment such as the Illumina NovaSeq 6000 to handle degraded or efficiently. As a private forensic laboratory, Othram collaborates exclusively with agencies on a case-by-case basis to generate investigative leads from biological evidence, without providing genetic testing services. Funding for its work derives from government grants, through the DNASolves platform—which enables public donations to specific cases—and private contributions, allowing the lab to prioritize unsolved violent crimes and unidentified remains. The operational workflow commences with submitting case details and via a secure online request system, which Othram reviews for feasibility before coordinating secure shipment to the lab for processing, including , library preparation, sequencing, and bioinformatics analysis. To build family trees for , the lab supplies at-home cheek swab kits to potential relatives, whose anonymized profiles are added to the private DNASolves database for matching. Othram maintains rigorous ethical standards in handling genetic data, complying with regulations through measures like data minimization, unidirectional workflows to prevent , and selective use of non-public databases for particularly sensitive investigations to safeguard third-party . Employing a multidisciplinary of 51 to 200 professionals, including geneticists, bioinformaticians, forensic analysts, and genealogists, Othram supports by processing hundreds of cases annually and has contributed to the resolution of thousands of cold cases worldwide, with over 600 publicly announced, as of November 2025.

Technology

DNA analysis methods

Traditional short tandem repeat () analysis, the standard method in forensic DNA testing for decades, faces significant limitations when dealing with degraded or low-quantity samples common in cold cases. STR profiling relies on amplifying longer DNA fragments (typically 100-500 base pairs), which often fail to yield complete profiles from environmentally exposed evidence like bones, , or historical remains, leading to inconclusive results or inability to perform . Othram addresses these challenges through advancements in tailored for forensics, enabling the generation of comprehensive DNA profiles from highly degraded samples that STR methods cannot process. Their approach shifts to sequencing (MPS) of shorter DNA fragments (as small as 50 base pairs), which are more likely to survive degradation, thus expanding the pool of solvable cases. Central to Othram's methodology is Forensic-Grade Sequencing (FGGS®), a process that achieves high-coverage sequencing from minimal input, such as as little as 15 cells or 0.1 nanograms of . FGGS incorporates specialized , enrichment via targeted amplification, chemical repair of damaged molecules, and advanced error-correction algorithms in the bioinformatics pipeline to ensure accurate reconstruction of the despite or fragmentation. This end-to-end workflow, performed in an ANSI-accredited ISO/IEC 17025:2017 laboratory, digitizes the full spectrum of for downstream analysis. In SNP testing, Othram captures hundreds of thousands of single nucleotide polymorphisms (SNPs), such as approximately 650,000, across the , far surpassing the 20-30 markers in STR profiles. These dense SNP datasets enable precise estimation of ancestry and distant relatedness by identifying shared genetic segments, providing a robust foundation for in forensic contexts. Sample preparation at Othram emphasizes handling challenging evidence types, including touch DNA from surfaces, skeletal remains, and ancient artifacts, through unidirectional workflows to prevent contamination and maintain chain-of-custody. For instance, in a demonstration case involving bones discovered in 2016 but linked to a death in 1881, Othram extracted and sequenced DNA from the degraded remains, successfully generating a profile that facilitated identification via genetic genealogy.

Genetic genealogy techniques

Othram applies sequenced DNA data to investigative genetic genealogy by first generating single nucleotide polymorphism (SNP) profiles containing hundreds of thousands of markers from forensic samples, enabling the detection of distant relatives such as third- or fourth-degree cousins. These profiles are then uploaded to opt-in public databases like GEDmatch and FamilyTreeDNA, where users have consented to law enforcement matching, allowing Othram to identify genetic matches through proprietary KinSNP® analysis. Pedigrees are constructed using algorithms that perform cousin matching by measuring shared DNA segments in centimorgans (cM) and automatically clustering matches based on overlapping identity-by-descent (IBD) regions to infer family relationships. To enhance profile utility, Othram employs phasing and imputation techniques, which reconstruct haplotypes by inferring missing genotypes from reference panels of known ancestries, thereby narrowing potential relatives and improving match compatibility with databases. of matches follows, confirming biological links by verifying shared segments across multiple individuals, which helps distinguish true relatives from coincidental matches and builds reliable family trees. While imputation fills gaps in sparse data, Othram prioritizes directly observed SNPs from high-coverage sequencing to minimize errors, particularly in cases involving degraded or mixed samples. In 2025, Othram introduced SNPSuite, a secure, offline desktop application designed for forensic laboratories to perform advanced SNP-based and comparisons without compromising . Othram's in-house team of genealogists collaborates on pedigree verification, cross-referencing genetic clusters with , vital statistics, and to trace lineages and identify candidates. This manual review integrates automated tools with traditional methods, such as analyzing files for relationship graphs, to resolve ambiguities and generate investigative leads for . Ethical protocols are integral to Othram's process, requiring explicit opt-in consent from database users for forensic searches, ensuring only profiles from consenting individuals are used for matching. Non-case data, including third-party reference profiles, is anonymized during analysis to protect , with results presented as hypothesis-testing frameworks rather than definitive identifications to uphold standards of genomic .

History and development

Establishment and early years

Othram was founded in 2018 by David Mittelman and Kristen Mittelman, drawing on their academic backgrounds in genomics from Baylor College of Medicine, where they had been developing advanced genomic techniques for analyzing degraded DNA samples. The company, headquartered in The Woodlands, Texas, initially concentrated on demonstrating the feasibility of its proprietary Forensic-Grade Genome Sequencing technology using historical samples, such as the successful identification of human remains dating back to 1881. This early work highlighted the potential of the method to handle extremely old and compromised evidence, setting the stage for forensic applications. In its formative phase, Othram faced significant challenges in refining its technology for low-quantity, degraded DNA while navigating limited funding available for forensic research and development. To overcome these hurdles, the company established initial partnerships with Texas law enforcement agencies, leveraging its proximity and expertise to test and validate its processes on real-world cases. These collaborations were crucial for building credibility and access to evidence, despite the field's traditional reliance on underfunded public labs. Breakthroughs emerged in 2019 when Othram assisted in the Christine Jessop murder investigation, where provided crime scene evidence for genetic analysis, leading to the eventual identification of the perpetrator through genealogical profiling. The company also began applying its methods to early unidentified remains cases, contributing to identifications that demonstrated the technology's reliability beyond standard DNA matching. Prior to 2020, Othram invested in constructing specialized laboratory infrastructure designed specifically for end-to-end forensic sequencing, including automated enrichment and unidirectional workflows to prevent . This expansion was supported by securing an initial $4 million Series A funding round in March 2019, which enabled scaling operations. Despite its for-profit structure, Othram adopted a mission-oriented approach akin to a non-profit, often subsidizing or funding cases to address funding gaps in investigations.

Expansion and milestones

During the early years of the in 2020 and 2021, Othram experienced significant growth in case submissions from agencies, driven by increased interest in advanced DNA technologies for cold cases amid disruptions to traditional investigative workflows. In , the company launched DNASolves, a platform that enables public contributions to fund forensic testing for unsolved cases, which gained traction during this period to support resource-limited agencies. Othram also forged key partnerships with federal entities, including the FBI's New York field office for victim identifications, and numerous state agencies to process evidence in high-profile investigations. A pivotal funding milestone came in September 2021, when Othram secured $18 million in Series B financing led by Gigafund, enabling expansion of its laboratory capacity and technology development to handle a broader range of forensic evidence. This investment supported subsequent initiatives, including the 2024 launch of Project 525, a targeted effort funded through donations to apply forensic-grade genome sequencing to 525 unidentified juvenile cases in the National Missing and Unidentified Persons System (NamUs), aiming to restore identities and advance related investigations. As of November 2025, Project 525 had resulted in at least nine identifications, contributing to Othram's estimated total of over 6,000 case resolutions since inception. By 2023, Othram's technologies had contributed to the resolution of over 1,500 cases annually, marking a substantial increase from earlier volumes in the dozens per year. From 2022 to 2025, Othram broadened its scope to include specialized work on Missing and Murdered Indigenous Persons (MMIP) cases through a 2025 partnership with the U.S. ' Missing and Murdered Unit under Operation Spirit Return, focusing on identifying remains and repatriating victims to tribal communities. The company also established international collaborations, such as with the Australian Federal Police's National DNA Program in 2022 for applications and with Humber College in to develop training tools for . Technological advancements during this period included the introduction of SNPSuite software in 2025 for deployable SNP analysis workflows and OthramOS, an AI-driven platform to accelerate evidence organization and kinship matching, reducing processing times for complex cases. Othram's overall quantitative growth reflected this expansion, with annual case resolutions scaling from limited numbers in 2020 to thousands by 2025, supported by over $48 million in total funding across multiple rounds. To foster ongoing innovation, the company established the Othram Fellowship Program, a paid 120-day initiative launched in 2024 to train researchers and law enforcement professionals in forensic genetic genealogy techniques.

Notable contributions

Identification of suspects in cold cases

Othram's approach to identifying suspects in cold cases relies on advanced to create detailed genetic profiles from collected at crime scenes, such as semen or from decades-old samples. These profiles are uploaded to genealogical databases to match with of potential perpetrators, after which and genealogists construct family trees to narrow down candidates. Confirmation occurs through direct DNA comparison from the suspect's relatives or the individual themselves, often leading to arrests or indictments long after the crimes. A prominent example is the 1974 abduction and murder of 17-year-old Carla Walker in Fort Worth, Texas. In 2020, the Fort Worth Police Department partnered with Othram to analyze DNA from Walker's clothing, resulting in the identification of 77-year-old Glen Samuel McCurley as the suspect. McCurley confessed during interrogation and was convicted of capital murder in 2021, receiving a life sentence. This breakthrough, achieved 46 years after the crime, highlighted Othram's collaboration with local agencies to revive stalled investigations. In the 1984 sexual assault and strangulation of nine-year-old Christine Jessop in Ontario, Canada, Othram's genetic genealogy work in 2020 identified Calvin Ray Hoover, who had died in 2015, as the true perpetrator. This revelation exonerated Guy Paul Morin, who had been wrongfully convicted and imprisoned for the crime in 1992 before his 1995 acquittal based on DNA evidence. The case underscored Othram's role in not only naming killers but also rectifying miscarriages of justice through precise DNA matching. Another significant identification occurred in the 1974 abduction, sexual assault, and of five-year-old Siobhan McGuinness in . Othram's analysis of evidence in 2020 pointed to , a transient who had passed away in 2012, as the culprit, closing the case after 46 years and providing closure to the family without the possibility of prosecution. More recently, in 2025, Othram assisted the Shasta County Sheriff's Office in with the 1984 shooting murder of 18-year-old Terrance Arndt and of his 15-year-old female companion near their high school in Burney. Forensic evidence submitted to Othram led to the identification of Roger Neil Schmidt as the suspect via genealogical matching. Schmidt was arrested on July 21, 2025, in , on murder and charges. By 2025, Othram had contributed to the identification of dozens of suspects in cold cases nationwide, predominantly involving homicides and sexual assaults, often resulting in arrests decades after the incidents and demonstrating the transformative impact of genetic genealogy on forensic investigations.

Resolution of unidentified remains cases

Othram's approach to resolving unidentified remains cases involves extracting and sequencing DNA from skeletal or degraded biological samples, generating a comprehensive genetic profile, and applying forensic genetic genealogy to construct family trees on public databases. This process identifies potential relatives through shared DNA segments, which are then verified via direct comparisons or law enforcement collaboration, ultimately confirming the decedent's identity and providing closure to families. The technology addresses significant challenges, such as degraded DNA from decades-old remains exposed to environmental factors like water, soil, or chemicals, where traditional methods often fail due to low quantities or contamination. For instance, Othram has successfully analyzed samples as small as 0.12 nanograms or from chemically damaged bones, enabling identifications in cases spanning 40-50 years. International cases, including those with non-U.S. origins, are handled by tracing global ancestry patterns and coordinating with foreign authorities. Key examples illustrate these successes. In 2020, Othram identified the remains of an 18-month-old girl found in Mississippi's Escatawpa River in 1982, known as "," as Alisha Ann Heinrich, matching her DNA to maternal relatives after prior tests yielded no results. That same year, the unidentified hiker found in Florida's in 2018, dubbed "," was named Vance Rodriguez through genealogy linking him to Louisiana family roots. In 2021, "Beth Doe," a pregnant teenager murdered in in 1976, was identified as Evelyn Colon, whose exhumed remains provided enough DNA for relative matches despite 45 years of degradation. Also in 2021, "Septic Tank Sam," whose burned body was discovered in an Alberta in 1977, was confirmed as Gordon Edwin Sanderson via sequencing that overcame charring and submersion effects. The 2022 identification of "Lady of the Dunes," a woman killed in in 1974, as Ruth Marie Terry relied on Othram's sequencing of chemically preserved tissue to build a viable . More recently, in October 2025, Othram identified remains found in 's Walnut Creek as Rebecca Hakes, a New York woman, resolving a case from 2024 and demonstrating applicability to fresher evidence. In November 2025, Othram identified remains found in Arizona's Pinal County in 2024 as David Bertschinger. These resolutions have facilitated family reunifications, allowing relatives to mourn and bury loved ones properly, while closing long-standing investigations and sometimes advancing probes. By November 2025, Othram had contributed to over 300 such identifications of unidentified human remains nationwide, underscoring their impact on forensic casework.

Impact and recognition

Influence on forensic science

Othram has pioneered the application of forensic-grade genome sequencing (FGGS), a next-generation sequencing approach that enables analysis of low-input and degraded DNA samples, significantly reducing reliance on traditional short tandem repeat (STR) profiling which often fails with compromised evidence. This shift to single nucleotide polymorphism (SNP) testing allows for the generation of comprehensive genetic profiles from as little as 1 nanogram of DNA, facilitating distant kinship inferences that were previously unattainable in forensic contexts. By developing purpose-built SNP tools for investigative genetic genealogy (IGG) since 2018, Othram has influenced law enforcement standards, with more cases resolved using their methods than any other forensic genetic genealogy approach, prompting updates to guidelines such as those from the U.S. Department of Justice on prioritizing SNP-based investigations. Othram's advocacy has driven policy changes to support expanded access to advanced DNA testing, including contributions to legislative efforts like the Carla Walker Act, which establishes federal grants for forensic in non-homicide s. In , Othram's work aligned with state initiatives securing $1.5 million in funding in 2024 through Rep. Tricia Byrnes's efforts, enabling the to process unidentified remains and resolve multiple cases in 2025 via FGGS. Additionally, Othram has partnered with national programs, such as the Australian Federal Police's National DNA Program, to integrate FGGS into broader database initiatives for unidentified remains and leads, enhancing cross-jurisdictional data sharing. Through platforms like DNASolves.com, Othram has also facilitated for SNP database development tailored to needs. Statistically, Othram's technology has contributed to the resolution of over 600 publicly announced cases across the U.S., as of November 2025, demonstrating its role in accelerating solves for longstanding cold cases that traditional methods could not address. This includes notable progress in Missing and Murdered Indigenous People (MMIP) investigations, where a 2025 partnership with the ' Missing and Murdered Unit has enabled to identify suspects and victims in historically under-resourced cases, such as those on the . Looking ahead, Othram's advancements in familial DNA searching—distinct from traditional IGG by focusing on close relatives in CODIS-like databases—hold potential for proactive crime prevention by generating leads in active investigations before cases go cold. To support this evolution, Othram offers training programs for agencies, including the 120-day Fellowship for forensic genetic genealogy immersion, beta access to secure software like SNPSuite with dedicated support, and sponsored workshops such as countywide sessions on DNA and familial leads.

Cultural and media references

Othram's advancements in forensic have inspired fictional portrayals in television, notably serving as the basis for the 500th episode of : , titled "The Five Hundredth Episode," which aired in October 2021 and dramatized the use of advanced to solve cases through familial matches. The episode fictionalized Othram's laboratory processes and team dynamics, highlighting the ethical tensions of genetic searching in criminal investigations. Additionally, Othram's work has been featured in documentaries and series, such as episodes of that explore DNA breakthroughs in unsolved murders, emphasizing the lab's role in reanalyzing degraded evidence to identify perpetrators decades later. Othram has garnered significant public awareness through extensive news coverage of its case resolutions, particularly in 2024 and 2025, with outlets like ABC13 Houston profiling the Woodlands-based lab's contributions to identifying victims and suspects in long-standing mysteries. For instance, a March 2025 ABC13 report detailed how Othram's sequencing technology resolved cases baffling investigators for years, including homicides and unidentified remains, while a related ABC News 20/20 segment in the same period showcased the lab's facility and its impact on law enforcement partnerships. Podcasts have further amplified this visibility; in October 2025, Criminally Obsessed hosted an interview with Othram co-founder and Chief Development Officer Kristen Mittelman, discussing the company's genealogy-driven approaches to casework and public involvement in DNA submissions. The rise of Othram's methods has sparked media debates on implications of familial DNA searching, particularly after high-profile cases where genetic profiles from public databases implicated relatives of suspects without their direct consent. Outlets like in highlighted concerns over the ethical boundaries of such searches, noting Othram's role in generating leads that extended beyond traditional databases and raised questions about and innocent family members' rights. These discussions have continued into 2025, with journalistic analyses framing Othram's successes as a double-edged sword in balancing justice with individual protections. Othram has received recognition for its forensic innovations through prestigious grants and contracts, underscoring its influence on the field. In June 2024, the Research Laboratory's AFWERX program awarded Othram a $1.25 million Phase II contract to enhance for identifying missing service members, building on an earlier Phase I award. Earlier accolades include a 2019 research sequencing grant from the Illumina Accelerator at SXSW, which supported Othram's development of biology-digitizing tools for forensics. In 2025 interviews, such as the Criminally Obsessed episode, Othram leaders elaborated on using preemptively to anticipate and prevent crimes by mapping familial risks and encouraging voluntary DNA uploads to aid investigations proactively. In November 2025, donated nearly $6,000 to Othram to assist in identifying human remains found in Eureka Springs, further demonstrating ongoing collaborations with state agencies.

References

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