2021 I.ČLTK Prague Open

Country	Player	Rank1	Seed
SVK	Norbert Gombos	95	1
JPN	Yūichi Sugita	109	2
SVK	Andrej Martin	110	3
AUT	Dennis Novak	111	4
USA	Denis Kudla	117	5
POL	Kamil Majchrzak	123	6
GER	Peter Gojowczyk	132	7
IND	Sumit Nagal	137	8

• ¹ Rankings are as of 26 April 2021.^[1]

The following players received wildcards into the singles main draw:

• Jiří Lehečka
• Andrew Paulson
• Michael Vrbenský

The following player received entry into the singles main draw as a special exempt:

• Renzo Olivo

The following players received entry from the qualifying draw:

• Zdeněk Kolář
• Vít Kopřiva
• Sergiy Stakhovsky
• Kacper Żuk

Country	Player	Rank1	Seed
SVK	Viktória Kužmová	106	1
ROU	Jaqueline Cristian	147	2
AUT	Barbara Haas	156	3
ROU	Elena-Gabriela Ruse	175	4
TUR	Çağla Büyükakçay	177	5
NED	Lesley Pattinama Kerkhove	179	6
SVK	Rebecca Šramková	196	7
AUT	Julia Grabher	197	8

• ¹ Rankings are as of 26 April 2021.

The following players received wildcards into the singles main draw:

• Nikola Bartůňková
• Ana Konjuh
• Linda Nosková
• Darja Viďmanová

The following player received entry using a protected ranking:

• Karman Thandi

The following players received entry from the qualifying draw:

• Anna Bondár
• Amanda Carreras
• Johana Marková
• Jule Niemeier
• Paula Ormaechea
• İpek Öz
• Chantal Škamlová
• Shalimar Talbi

• Tallon Griekspoor def. Oscar Otte 5–7, 6–4, 6–4.

• Jule Niemeier def. Dalma Gálfi, 6–4, 6–2

• Marc Polmans / Sergiy Stakhovsky def. Ivan Sabanov / Matej Sabanov 6–3, 6–4.

• Anna Bondár / Kimberley Zimmermann def. Xenia Knoll / Elena-Gabriela Ruse, 7–6^(7–5), 6–2