Matthew 27 is the 27th chapter in the Gospel of Matthew, part of the New Testament in the Christian Bible. This chapter contains Matthew's record of the day of the trial, crucifixion and burial of Jesus. Scottish theologian William Robertson Nicoll notes that "the record of this single day is very nearly one-ninth of the whole book".

The original text was written in Koine Greek. This chapter is divided into 66 verses.

Some early manuscripts containing the text of this chapter are:

The New International Version (NIV) organises the material in this chapter as follows:

During the morning after his arrest, the trial of Jesus before the Sanhedrin is concluded with plans to have Jesus executed (verse 1), and he is taken to Pontius Pilate, the Roman governor (procurator) of Judea. As Jesus was being led away, Judas Iscariot, who had betrayed Jesus, sees that his former teacher has been condemned, and is overcome by remorse: in the words of the King James Version, he "repented himself". The word translated as "repented" (Greek: μεταμεληθεις, metamelētheis) is not the same as the word for repentance which John the Baptist and Jesus himself used in their ministry (Greek: μετανοειτε, metanoeite); Arthur Carr, in the Cambridge Bible for Schools and Colleges notes that "it implies no change of heart or life, but merely remorse or regret".

Judas brings back the 30 pieces of silver which had been given to him by the priests of Judea as recompense for identifying his master to Caiaphas, throwing them down in the temple, and then leaves to commit suicide. Meanwhile, Jesus impresses Pilate, who is taken aback by Jesus' silent dignity at his questioning over the "many charges" brought against him. Pilate begins to address the crowd, and knowing (or "shrewdly suspecting") that the chief priests had handed Jesus over because they were jealous of his popularity, asks the crowd to choose between freeing a notorious prisoner known as Barabbas, or Jesus. The crowd, persuaded by the chief priests and elders, respond passionately, repeating "Let Him (Christ) be crucified!" Pilate, bewildered by this, asks the crowd for a reason for their choice. Instead, they continue to call ever more loudly for the crucifixion of Jesus.

Pilate comes to see that he cannot reason with the crowd. His wife has had a disturbing dream and asks him to have "nothing to do with that just man". Instead, he tries to absolve himself of his responsibility in the case, washing his hands in a basin and saying to the crowd: "I am innocent of the blood of this just Person. You see to it". Then the Jews present at the trial take responsibility for the shedding of Jesus' blood. Pilate has Barabbas released, lets Jesus be flogged and sends him off to be crucified.

Jesus is led away to the Praetorium of the Governor's Residence, where Pilate's guard and the praetorian guard mock him, giving him a scarlet robe in place of his own clothes, a reed to hold as a sign of his "kingship" and a crown made of twisted thorns. The soldiers then replace the robe with Jesus' own clothes and lead him to Golgotha (the "place of a skull"); in Luke's Gospel this journey is recorded with "several particulars of what happened on the way to Golgotha, omitted in the other Gospels: the great company of people and of women who followed Him; the touching address of Jesus to the women; the last warning of the coming sorrows; the leading of two malefactors with Him". A man named Simon, from Cyrene, is compelled to carry Jesus' cross. At Golgotha he is offered wine mingled with gall, which he tastes but does not drink. The soldiers cast lots for his garments once he is crucified. Those who passed him deride him, taunting him to come down from the cross, saying "He trusts in God, let God deliver him now".