The Mohorovičić discontinuity (/ˌmoʊhəˈroʊvɪtʃɪtʃ/ MOH-hə-ROH-vih-chitch; Croatian: [moxorôʋiːtʃitɕ]) – usually called the Moho discontinuity, Moho boundary, or just Moho – is the boundary between the crust and the mantle of Earth. It is defined by the distinct change in velocity of seismic waves as they pass through changing densities of rock.

The Moho lies almost entirely within the lithosphere (the hard outer layer of the Earth, including the crust). Only beneath mid-ocean ridges does it define the lithosphere–asthenosphere boundary (the depth at which the mantle becomes significantly ductile). The Mohorovičić discontinuity is 5 to 10 kilometres (3–6 mi) below the ocean floor, and 20 to 90 kilometres (10–60 mi) beneath typical continental crusts, with an average of 35 kilometres (22 mi).

Named after the pioneering Croatian seismologist Andrija Mohorovičić, the Moho separates both the oceanic crust and continental crust from the underlying mantle. The Mohorovičić discontinuity was first identified in 1909 by Mohorovičić, when he observed that seismograms from shallow-focus earthquakes had two sets of P-waves and S-waves, one set that followed a direct path near the Earth's surface and the other refracted by a high-velocity medium.

The Moho marks the transition in composition between the Earth's crust and the lithospheric mantle. Immediately above the Moho, the velocities of primary seismic waves (P-waves) are consistent with those through basalt (6.7–7.2 km/s), and below they are similar to those through peridotite or dunite (7.6–8.6 km/s). This increase of approximately 1 km/s corresponds to a distinct change in material as the waves pass through the Earth, and is commonly accepted as the lower limit of the Earth's crust. The Moho is characterized by a transition zone of up to 500 meters. Ancient Moho zones are exposed above-ground in numerous ophiolites around the world.

Beginning in the 1980s, geologists became aware that the Moho does not always coincide with the crust-mantle boundary defined by composition. Xenoliths (lower crust and upper mantle rock brought to the surface by volcanic eruptions) and seismic-reflection data showed that, away from continental cratons, the transition between crust and mantle is marked by basaltic intrusions and may be up to 20 km thick. The Moho may lie well below the crust-mantle boundary and care must be used in interpreting the structure of the crust from seismic data alone.

Serpentinization of mantle rock below slowly spreading mid-ocean ridges can also increase the depth to the Moho, since serpentinization lowers seismic wave velocities.

Croatian seismologist Andrija Mohorovičić is credited with discovering and defining the Moho. In 1909, he was examining data from a local earthquake in Zagreb when he observed two distinct sets of P-waves and S-waves propagating out from the focus of the earthquake. Mohorovičić knew that waves caused by earthquakes travel at velocities proportional to the density of the material carrying them. As a result of this information, he theorized that the second set of waves could only be caused by a sharp transition in density in the Earth's crust, which could account for such a dramatic change in wave velocity. Using velocity data from the earthquake, he was able to calculate the depth of the Moho to be approximately 54 km, which was supported by subsequent seismological studies.

The Moho has played a large role in the fields of geology and earth science for well over a century. By observing the Moho's refractive nature and how it affects the speed of P-waves, scientists were able to theorize about the earth's composition. These early studies gave rise to modern seismology.