An autotroph is an organism that can convert abiotic sources of energy into energy stored in organic compounds, which can be used by other organisms. Autotrophs produce complex organic compounds (such as carbohydrates, fats, and proteins) using carbon from simple substances such as carbon dioxide, generally using energy from light or inorganic chemical reactions. Autotrophs do not need a living source of carbon or energy and are the producers in a food chain, such as plants on land or algae in water. Autotrophs can reduce carbon dioxide to make organic compounds for biosynthesis and as stored chemical fuel. Most autotrophs use water as the reducing agent, but some can use other hydrogen compounds such as hydrogen sulfide.

The primary producers can convert the energy in the light (phototroph and photoautotroph) or the energy in inorganic chemical compounds (chemotrophs or chemolithotrophs) to build organic molecules, which is usually accumulated in the form of biomass and will be used as carbon and energy source by other organisms (e.g. heterotrophs and mixotrophs). The photoautotrophs are the main primary producers, converting the energy of the light into chemical energy through photosynthesis, ultimately building organic molecules from carbon dioxide, an inorganic carbon source. Examples of chemolithotrophs are some archaea and bacteria (unicellular organisms) that produce biomass from the oxidation of inorganic chemical compounds; these organisms are called chemoautotrophs, and are frequently found in hydrothermal vents in the deep ocean. Primary producers are at the lowest trophic level, and are the reasons why Earth sustains life to this day.

Autotrophs use a portion of the ATP produced during photosynthesis or the oxidation of chemical compounds to reduce NADP⁺ to NADPH to form organic compounds. Most chemoautotrophs are lithotrophs, using inorganic electron donors such as hydrogen sulfide, hydrogen gas, elemental sulfur, ammonium and ferrous oxide as reducing agents and hydrogen sources for biosynthesis and chemical energy release. Chemolithoautotrophs are microorganisms that synthesize energy through the oxidation of inorganic compounds. They can sustain themselves entirely on atmospheric CO₂ and inorganic chemicals without the need for light or organic compounds. They enzymatically catalyze redox reactions using mineral substrates to generate ATP energy. These substrates primarily include hydrogen, iron, nitrogen, and sulfur. Its ecological niche is often specialized to extreme environments, including deep marine hydrothermal vents, stratified sediment, and acidic hot springs.

The term autotroph was coined by the German botanist Albert Bernhard Frank in 1892. It stems from the ancient Greek word τροφή (trophḗ), meaning "nourishment" or "food". The first autotrophic organisms likely evolved early in the Archean but proliferated across Earth's Great Oxidation Event with an increase to the rate of oxygenic photosynthesis by cyanobacteria. Photoautotrophs evolved from heterotrophic bacteria by developing photosynthesis. The earliest photosynthetic bacteria used hydrogen sulphide. Due to the scarcity of hydrogen sulphide, some photosynthetic bacteria evolved to use water in photosynthesis, leading to cyanobacteria.

Some organisms rely on organic compounds as a source of carbon, but are able to use light or inorganic compounds as a source of energy. Such organisms are mixotrophs. An organism that obtains carbon from organic compounds but obtains energy from light is called a photoheterotroph, while an organism that obtains carbon from organic compounds and energy from the oxidation of inorganic compounds is termed a chemolithoheterotroph.

Evidence suggests that some fungi may also obtain energy from ionizing radiation: Such radiotrophic fungi were found growing inside a reactor of the Chernobyl nuclear power plant.

There are many different types of autotrophs in Earth's ecosystems. Lichens located in tundra climates are an exceptional example of a primary producer that, by mutualistic symbiosis, combines photosynthesis by algae (or additionally nitrogen fixation by cyanobacteria) with the protection of a decomposer fungus. As there are many examples of primary producers, two dominant types are coral and one of the many types of brown algae, kelp.

Gross primary production occurs by photosynthesis. This is the main way that primary producers get energy and make it available to other forms of life. Plants, many corals (by means of intracellular algae), some bacteria (cyanobacteria), and algae do this. During photosynthesis, primary producers receive energy from the sun and use it to produce sugar and oxygen.