Jones was born in Hammersmith, London^[3] to actors Jennifer Jones (née Heslewood) and Freddie Jones, and grew up in Oxford.^[4][5] He has two brothers.^[6] He attended Christ Church Cathedral School and Abingdon School in Oxfordshire in the 1980s. He studied drama at the University of Manchester from 1986 to 1989, and at L'École Internationale de Théâtre Jacques Lecoq in Paris from 1989 to 1991.^[4]

Jones has appeared in more than 60 films since his first acting role in the 1992 film Orlando. He voiced Dobby in two Harry Potter films: Chamber of Secrets (2002) and The Deathly Hallows – Part 1 (2010). He played Robert Cecil, 1st Earl of Salisbury in the HBO/Channel 4 production Elizabeth I. In 2006, he portrayed Truman Capote in the biopic Infamous. He appeared in the film adaptation of Stephen King's The Mist in 2007. In 2008, he portrayed Karl Rove in Oliver Stone's W and Hollywood agent Swifty Lazar in Frost/Nixon. He appeared alongside his father in the 2004 film Ladies in Lavender.

Jones appeared in the 2010 episode "Amy's Choice", of Doctor Who, as the Dream Lord, and in the Big Finish Productions series' Dark Eyes (audio drama) as Kotris. He also played the role of Samuel Ratchett in Agatha Christie's Poirot TV Series 12 episode "Murder on the Orient Express". In 2011, he played the role of the British spy master Percy Alleline in the adaptation of John Le Carré's Tinker Tailor Soldier Spy and Arnim Zola in Captain America: The First Avenger, a role which he reprised in the sequel Captain America: The Winter Soldier three years later as well as in a cameo in the TV series Agent Carter the following year. In 2012, he had a leading role in the ITV mini-series Titanic, starred as one of the seven dwarves in Snow White and the Huntsman, played Dr. Paul Shackleton in Red Lights, and Max in Virginia. He also portrayed film director Alfred Hitchcock in the HBO television film The Girl, a role that earned him his first Golden Globe Award nomination, as well as his first Primetime Emmy Award nomination.

In 1998, he appeared as a City businessman in the music video for Gomez's song "Whippin' Piccadilly", from their album Bring It On.^[7]

He played Neil Baldwin in the BBC drama Marvellous in 2014.^{[8][9][10][11][12][5]} Sam Wollaston, in The Guardian, praised Jones's "lovely, very human, performance",^[13] one that earned him his second British Academy Television Award nomination. From 2014, he appeared in the BBC Four television series Detectorists,^[14] for which he received a nomination for the British Academy Television Award for Best Male Comedy Performance in 2016 before winning the award in 2018.

In 2015, Jones played the part of Roger Yount, a banker, in the three-part BBC series Capital based on John Lanchester's novel of the same name.^[15] Discussing working with Jones on Capital, writer Peter Bowker said, "I think Toby is a genius and thought that long before I worked with him. He always wants to know a character's needs, and what's beneath those needs. Then he takes all that material and somehow embeds it into the character and physically inhabits the character, so that you never think he's playing the character. It's fascinating to watch him close up. He carries the emotional complexities in every tiny gesture that his character makes so that you immediately can see what his character is like. A character like Roger is full of contradictions, a city banker with an air of entitlement but also a little insecurity picking away at him. Toby can portray that in his walk alone. That's what's great about him, he can portray cold he can portray warm and he can portray both of those things at once."^[16]

He played Captain Mainwaring in the film Dad's Army, released in February 2016.^[17] In July, of the same year he starred as the eponymous agent Verloc in the BBC's The Secret Agent, a three-part television adaptation of Joseph Conrad's 1907 novel.^[18]

In 2017, he portrayed Culverton Smith in "The Lying Detective", an episode of the BBC crime drama Sherlock. In 2018, he played the dinosaur auctioneer Mr. Eversoll in Jurassic World: Fallen Kingdom, the fifth instalment of the Jurassic Park series.^[19] In the same year, Jones voiced Owl in Disney's live-action Christopher Robin.

Jones co-wrote with Tim Crouch the comedy series Don't Forget the Driver, released on BBC 2 in 2019, and in which he appeared as Peter Green; it was Jones's first time writing for television.^[3][20]

In 2024, Jones played Alan Bates in the ITV drama Mr Bates vs The Post Office, a dramatisation of the British Post Office scandal.

He appears as newspaper editor Alan Rusbridger in ITV television drama series about the News International phone hacking scandal, The Hack.^[21]

In 2003 Jones played the part of Lord Brideshead in a BBC Radio adaptation of Brideshead Revisited. Jones voiced the title character in the 2005 BBC Radio 4 adaptation of Oblomov. He also read the 2009 Radio 4 adaptation of John Irving's A Prayer for Owen Meany. He played Inspector Goole in the 2010 BBC Radio adaptation of An Inspector Calls. Since 2013 Jones has been the voice of the lead character, Joey Oldman in the BBC Radio 4 series The Corrupted, an adaptation of the G. F. Newman novel Crime and Punishment. On 2 December 2012 he played Napoleon Bonaparte in Anthony Burgess's Napoleon Rising on Radio 3. In 2013 he played Kotris in the award-winning Doctor Who audio play, Dark Eyes, and read an abridged version of "The Manual of Detection" by Jedediah Berry for the BBC.^[22] In 2020 he portrayed Falstaff in BBC Radio 3's Henry IV, Part 1.^[23]

In 2021, Jones recorded the audiobook versions of John Le Carré's final novel Silverview for Penguin Audio and, for Harper Audio, the seminal dystopian novel We by Yevgeny Zamyatin in a translation by Bela Shayevich.

In 2001, he starred in the London West End comedy The Play What I Wrote, directed by Kenneth Branagh. His comic turn as Arthur earned him the Olivier Award for Best Actor in a Supporting Role, and the play moved to Broadway in 2003.

In 2009, he returned to the stage in Every Good Boy Deserves Favour at the National Theatre,^[24] Parlour Song at the Almeida Theatre,^[25] and The First Domino at Brighton Festival Fringe.^[26] In 2011, he played J. M. W. Turner in The Painter at the Arcola Theatre. Jones starred as Stanley in the 2018 revival of The Birthday Party at The Harold Pinter Theatre.^[27] In 2020, he starred in the title role in the Conor McPherson adaptation of Uncle Vanya by Anton Chekhov, at the Harold Pinter Theatre.

On The Graham Norton Show, Jones said that he and his wife Karen were together for 26 years before they married in 2015. They have two daughters.^[28]

In 2018, Jones was awarded an honorary doctorate by Oxford Brookes University.^[29] He was appointed Officer of the Order of the British Empire (OBE) in the 2021 New Year Honours, for services to drama.^[30]

In July 2025, Jones was awarded an honorary degree by Keele University.^[48] At the award ceremony he was reunited with Neil Baldwin, whom he played in Marvellous.^[48][49]

• List of Old Abingdonians