Myrmicinae is a subfamily of ants, with about 140 extant genera; their distribution is cosmopolitan. The pupae lack cocoons. Some species retain a functional sting. The petioles of Myrmicinae consist of two nodes. The nests are permanent and in soil, rotting wood, under stones, or in trees. All species of Cephalotes (within the tribe Attini) are gliding ants.

Myrmicine worker ants have a distinct postpetiole, i.e., abdominal segment III is notably smaller than segment IV and set off from it by a well-developed constriction; the pronotum is inflexibly fused to the rest of the mesosoma, such that the promesonotal suture is weakly impressed or absent, and a functional sting is usually present. The clypeus is well-developed; as a result, the antennal sockets are well separated from the anterior margin of the head. Most myrmicine genera possess well-developed eyes and frontal lobes that partly conceal the antennal insertions.

Recently, the number of tribes was reduced from 25 to six:

In 2014, most genera were placed into different tribes or moved to other subfamilies. Below is an updated list: