PCI-X, short for Peripheral Component Interconnect eXtended, is a computer bus and expansion card standard that enhances the 32-bit PCI local bus for higher bandwidth demanded mostly by servers and workstations. It uses a modified protocol to support higher clock speeds (up to 133 MHz), but is otherwise similar in electrical implementation. PCI-X 2.0 added speeds up to 533 MHz, with a reduction in electrical signal levels.

The slot is physically a 3.3 V PCI slot, with the same size, location and pin assignments. The electrical specifications are compatible, but stricter. However, while most conventional PCI slots are the 85 mm long 32-bit version, most PCI-X devices use the 130 mm long 64-bit slot, to the point that 64-bit PCI connectors and PCI-X support are seen as synonymous.

PCI-X is specified for both 32- and 64-bit PCI connectors, and PCI-X 2.0 added a 16-bit variant for embedded applications.

PCI-X has been replaced in modern designs by the similar-sounding PCI Express (PCIe), with a different physical connector and a different electrical design, having one or more serial lanes instead of a number of slower parallel connections.

In PCI, a transaction that cannot be completed immediately is postponed by either the target or the initiator issuing retry-cycles, during which no other agents can use the PCI bus. Since PCI lacks a split-response mechanism to permit the target to return data at a later time, the bus remains occupied by the target issuing retry-cycles until the read data is ready. In PCI-X, after the master issues the request, it disconnects from the PCI bus, allowing other agents to use the bus. The split-response containing the requested data is generated only when the target is ready to return all of the requested data. Split-responses increase bus efficiency by eliminating retry-cycles, during which no data can be transferred across the bus.

PCI also suffered from the relative scarcity of unique interrupt lines. With only 4 interrupt pins (INT A/B/C/D), systems with many PCI devices require multiple functions to share an interrupt line, complicating host-side interrupt-handling. PCI-X added Message Signaled Interrupts, an interrupt system using writes to host-memory. In MSI-mode, the function's interrupt is not signaled by asserting an INTx line. Instead, the function performs a memory-write to a system-configured region in host-memory. Since the content and address are configured on a per-function basis, MSI-mode interrupts are dedicated instead of shared. A PCI-X system allows both MSI-mode interrupts and legacy INTx interrupts to be used simultaneously (though not by the same function).

The lack of registered I/Os limited PCI to a maximum frequency of 66 MHz. PCI-X I/Os are registered to the PCI clock, usually through means of a PLL to actively control I/O delay the bus pins. The improvement in setup time allows an increase in frequency to 133 MHz.

Some devices, most notably Gigabit Ethernet cards, SCSI controllers (Fibre Channel and Ultra320), and cluster interconnects could by themselves saturate the PCI bus's 133 MB/s bandwidth. Ports using a bus speed doubled to 66 MHz and a bus width doubled to 64 bits (with the pin count increased to 184 from 124), in combination or not, have been implemented. These extensions were loosely supported as optional parts of the PCI 2.x standards, but device compatibility beyond the basic 133 MB/s continued to be difficult.