Biloela (/bɪloʊˈiːlə/ BIL-oh-EE-lə) is a rural town and locality in the Shire of Banana, Central Queensland, Australia. It is the administrative centre of the shire. In the 2021 census, the locality of Biloela had a population of 5,692. The local high school won national fame on account of its dinosaur display and specimen with 66 footprints.

Biloela is 120 kilometres (75 mi) inland from the port city of Gladstone at the junction of the Burnett and Dawson highways. Biloela is the administrative centre of the Shire of Banana, which has an area of 28,610 square kilometres (11,050 sq mi).

The town was established on what is Gangulu tribal lands. Gangalu (Gangulu, Kangulu, Kanolu, Kaangooloo, Khangulu) is an Australian Aboriginal language spoken on Gangula country. The Gangula language region includes the towns of Clermont and Springsure extending south towards the Dawson River. There was a ceremonial bora ground behind what is now the main street of Bileola and the local entombment custom was to place the skeletal remains of their dead in hollowed out burial trees which were specially marked with red ochre. Dingoes were used in the process of mustering and killing of kangaroo and emu for food.

British colonisation began in 1854 when Frederick Morton established a large squatting pastoral property in the area, which he named Prairie. This leasehold comprised around 500 square miles of land in the Callide valley and Morton built his homestead not far from the present day location of the town of Biloela. Morton initially ran Prairie as a sheep station but later it was used to farm cattle.

In 1864, Morton decided to "disperse" a group of Aboriginal people for the taking of some sheep. He and other local settlers, armed and mounted on horses, set off on a night-time attack on a local Aboriginal camp. The people in the camp were made aware of the oncoming horsemen and set up an ambush. Morton's group was either warned at the last minute of the impending ambush or, according to historian John Bird, they were beaten back by the Aboriginal counter-attack and forced to retreat.

In 1873, a Native Police detachment of Alexander Douglas was accused of a massacre of an unknown number of Aboriginal people. An enquiry with several witnesses found that Douglas and his troopers had shot an Aboriginal man named Harry, who was shot while evading capture on a warrant for an "outrage" on a woman. The inquiry exonerated Douglas from the charge of wantonly destroying life. The local colonists signed a petition for him to conduct further patrols. An Aboriginal survivor of Douglas' raids named Etamitcham later described how as a child he and his family were chased over the Kroombit Mountains to avoid "being shot down". Aboriginal people were employed on Prairie Station and they considered themselves to be "well compensated" by being paid with trinkets and tobacco.

In 1886, most of Prairie was subdivided and sold off, with Montague Beak coming into ownership of what remained. Prairie was then resumed by the government in 1925 and completely divided into small land selections for urban development.

The name Biloela is generally believed to come from an Aboriginal word (possibly from the Sydney area) for "white cockatoo". The Government dockyards in Sydney were known as Biloela during 1871–1913 in an endeavour to remove the perceived stigma of the prior Cockatoo Island convict establishment.