A decimal separator is a symbol that separates the integer part from the fractional part of a number written in decimal form. Different countries officially designate different symbols for use as the separator. The choice of symbol can also affect the choice of symbol for the thousands separator used in digit grouping.

Any such symbol can be called a decimal mark, decimal marker, or decimal sign. Symbol-specific names are also used; decimal point and decimal comma refer to a dot (either baseline or middle) and comma respectively, when it is used as a decimal separator; these are the usual terms used in English, with the aforementioned generic terms reserved for abstract usage.

In many contexts, when a number is spoken, the function of the separator is assumed by the spoken name of the symbol: comma or point in most cases. In some specialized contexts, the word decimal is instead used for this purpose (such as in International Civil Aviation Organization-regulated air traffic control communications). In mathematics, the decimal separator is a type of radix point, a term that also applies to number systems with bases other than ten.

In the Middle Ages, before printing, a bar ( ¯ ) over the units digit was used to separate the integral part of a number from its fractional part, as in 9995 (meaning 99.95 in decimal point format). A similar notation remains in common use as an underbar to superscript digits, especially for monetary values without a decimal separator, as in 99⁹⁵. Later, a "separatrix" (i.e., a short, roughly vertical ink stroke) between the units and tenths position became the norm among Arab mathematicians (e.g. 99ˌ95), while an L-shaped or vertical bar (|) served as the separatrix in England. When this character was typeset, it was convenient to use the existing comma (99,95) or full stop (99.95) instead.

Positional decimal fractions appear for the first time in a book by the Arab mathematician Abu'l-Hasan al-Uqlidisi written in the 10th century. The practice is ultimately derived from the decimal Hindu–Arabic numeral system used in Indian mathematics, and popularized by the Persian mathematician Al-Khwarizmi, when Latin translation of his work on the Indian numerals introduced the decimal positional number system to the Western world. His Compendious Book on Calculation by Completion and Balancing presented the first systematic solution of linear and quadratic equations in Arabic.

Gerbert of Aurillac marked triples of columns with an arc (called a "Pythagorean arc"), when using his Hindu–Arabic numeral-based abacus in the 10th century. Fibonacci followed this convention when writing numbers, such as in his influential work Liber Abaci in the 13th century.

The earliest known record of using the decimal point is in the astronomical tables compiled by the Italian merchant and mathematician Giovanni Bianchini in the 1440s.^{[contradictory]}

Tables of logarithms prepared by John Napier in 1614 and 1619 used the period (full stop) as the decimal separator, which was then adopted by Henry Briggs in his influential 17th century work.