Toei Animation Co., Ltd. (Japanese: 東映アニメーション株式会社, Hepburn: Tōei Animēshon Kabushiki-gaisha; /ˈtoʊ.eɪ/) is a Japanese animation studio primarily controlled by its namesake Toei Company. It was originally founded on January 23, 1948, as Japan Animated Films by Kenzō Masaoka and Sanae Yamamoto.

The studio is known for producing numerous series, including the Sally the Witch series, the GeGeGe no Kitarō series, Mazinger Z, Galaxy Express 999, the Cutie Honey series, the Dr. Slump series, the Dragon Ball series, the Saint Seiya series, the Sailor Moon series, Slam Dunk, the Digimon series, the One Piece series, Magical Doremi, Toriko, World Trigger and the Pretty Cure series, among others. Aside from animation production, the company handles character licensing and overseas distribution and sales of its titles through its wholly-owned international subsidiaries.

Toei Animation is headquartered in Nakano, Tokyo with its studios in Higashiōizumi, Nerima, Tokyo which also houses the Toei Animation Museum [ja]. Toei also has an overseas studio in the Philippines known as Toei Animation Phils.

Pero, the protagonist of the studio's 1969 film adaptation of Puss in Boots, serves as its mascot.

The studio was founded by animators Kenzō Masaoka and Sanae Yamamoto in 1948 as Japan Animated Films (日本動画映画, Nihon Dōga Eiga) often shortened to Nichidō Eiga (日動映画). In 1956, Toei purchased the studio and it was renamed Toei Animation Co., Ltd. (東映動画株式会社, Tōei Dōga Kabushiki-gaisha; "dōga" is Japanese for "video", and in some extent "animation"). In 1998, the Japanese name was renamed to match with the English name. It has created a number of TV series and movies and adapted Japanese comics as animated series, many popular worldwide. Hayao Miyazaki, Isao Takahata, Yasuji Mori, Leiji Matsumoto and Yōichi Kotabe have worked with the company. Toei was a shareholder in the Japanese anime satellite television network Animax with other anime studios and production companies, such as Sunrise, TMS Entertainment and Nihon Ad Systems Inc.

Although the Toei Company usually contracts Toei Animation to handle its animation internally for some of their works, they occasionally hire other companies to provide animation; although the Toei Company produced the Robot Romance Trilogy, Sunrise (then known as Nippon Sunrise) provided the animation. Toei Company would also enlist the help of other studios such as hiring Academy Productions to produce the animation for Space Emperor God Sigma, rather than use their own studio. Since 1962, Toei Animation uses its own production offices rather than using Toei's television division.

Toei Animation's anime which have won the Animage Anime Grand Prix award are Galaxy Express 999 in 1981, Saint Seiya in 1987 and Sailor Moon in 1992. In addition to producing anime for release in Japan, Toei Animation began providing animation for American films and television series during the 1960s and particularly during the 1980s.

In October 2021, Toei Animation announced that they had signed a strategic partnership with the South Korean entertainment conglomerate CJ ENM.